植物遗传资源学报2025，Vol.26Issue(11)：2145-2163,19.DOI:10.13430/j.cnki.jpgr.20250218002
福建菜用大豆审定品种遗传多样性分析和SSR标记指纹图谱构建
Genetic Diversity Analysis and SSR Markers Fingerprint Construction of Vegetable Soybean Varieties in Fujian Province
摘要
Abstract
In this study,genetic diversity analysis and the construction of a molecular fingerprinting map were performed for 22 vegetable soybean varieties approved in Fujian province from 2003 to 2022,basing on 26 phenotypic traits and 30 pairs of SSR molecular markers.The results revealed there exhibited 16 types of variations in the seven qualitative traits among the 22 vegetable soybean varieties,with an average diversity index of 0.494.The variation coefficients of the 19 quantitative traits ranged from 3.44％to 50.60％,with an average diversity index of 1.934,indicating rich phenotypic genetic diversity.A total of 122 alleles were detected with 30 pairs of SSR molecular markers,the average number of alleles and effective number of alleles were 4.0667 and 2.7013.The average Nei's gene diversity index was 0.5691,and the average Shannon's polymorphism information index was 1.0479.The polymorphism information content varied from 0.0830 to 0.7319,with an average of 0.5262.The Satt184 maker demonstrated the highest polymorphism information content.The genetic similarity coefficient among the 22 varieties ranged from 0.3301 to 1.0000,with an average of 0.6341.The highest genetic similarity coefficient was observed between Huxuan 23-9 and Maodou 389,whereas the lowest coefficient was found between Qingshu No.6 and Mindou No.1.Cluster analysis showed that the 22 vegetable soybean cultivars were classified into three groups based on phenotypic characters and molecular markers.Mindou No.1 was classified into a separate group using both methods,indicating that it would have significant differences from other cultivars in both phenotypic traits and genetic background,making it suitable as a parental linefor hybridization.Considering the principles of uniform chromosomal distribution,distinct band patterns,and rich polymorphism,nine SSR makers(Satt197,Satt442,Satt268,Satt005,Satt431,Satt334,Satt556,Sat_1 12 and Satt487)were selected to construct the molecular fingerprint map of the 22 vegetable soybean varieties approved in Fujian province.With these primer combination,19 out of the 22 varieties could be distinguished,except for Huxuan 23-9,Maodou 389 and Zhexian No.10.The findings would provide a scientific foundation for the efficient utilization of vegetable soybean germplasm resources and breeding in Fujian province.This would also contribute to the differentiation and preservation of approved varieties.关键词
菜用大豆/分子标记/遗传多样性/指纹图谱Key words
vegetable soybean/molecular marker/genetic diversity/fingerprint引用本文复制引用
李清华,颜墩炜,刘雨杭,陈盈妹,顾智炜,陈子琳,林海峰,柯庆明,贾琪..福建菜用大豆审定品种遗传多样性分析和SSR标记指纹图谱构建[J].植物遗传资源学报,2025,26(11):2145-2163,19.基金项目
国家自然科学基金面上项目(32372061) （32372061）
福建省科技计划项目—农业引导性(2021N0039) （2021N0039）
福建省科技计划项目—星火项目(2023S0013) （2023S0013）
福建省自然科学基金面上项目(2024J01380) （2024J01380）
莆田市科技计划项目—技术开发与应用(2023NJJ002)General Program of National Natural Science Foundation of China(32372061) （2023NJJ002）
Fujian Provincial Science and Technology Project-Agricultural Guidance(2021N0039) （2021N0039）
Fujian Provincial Science and Technology Project-Spark Initiative(2023S0013) （2023S0013）
General Program of the Natural Science Foundation of Fujian Province(2024J01380) （2024J01380）
Putian Science and Technology Project-Technology Development and Application(2023NJJ002) （2023NJJ002）
