植物遗传资源学报2025，Vol.26Issue(11)：2193-2199,7.DOI:10.13430/j.cnki.jpgr.20250608001
水稻垩白和直链淀粉含量QTL qCR5/qAC5的定位和效应验证
Mapping and Effect Validation of QTL qCR5/qAC5 for Grain Chalkiness and Amylose Content in Rice
摘要
Abstract
To identify quantitative trait loci(QTL)associated with rice grain appearance and cooking/eating quality,a recombinant inbred line(RIL)population consisting of 181 lines was developed by crossing indica rice 9311 and tropical japonica rice Cypress.A high-density genetic linkage map was constructed using resequencing data,and QTL mapping was performed based on evaluations of chalky grain rate,chalkiness degree,and amylose content in 2018 and 2019.A total of four QTLs for chalky grain rate,five QTLs for chalkiness degree,and six QTLs for amylose content were detected.A novel locus qCR5/qAC5,simultaneously affecting chalky grain rate and amylose content,was consistently detected within the chromosomal interval 18397268-20398241 bp on chromosome 5.In 2018 and 2019,the LOD values for chalky grain rate were 3.11 and 3.82 and the additive effects were-5.54 and-6.12,respectively.In 2018 and 2019,the LOD values for amylose content were 3.76 and 3.82,and the additive effects were-1.06 and-1.05,respectively.And the 9311 genotype of qCR5/qAC5 simultaneously increased both chalky grain rate and amylose content.Near-isogenic lines(NILs)with the 9311 genetic background were developed through backcrossing,and the genetic effects of qCR5/qAC5 were validated using BC5F2 segregation population.This locus accounted for 20.9％of the phenotypic variation in chalky grain rate and 18.6％in amylose content.These findings lay a solid foundation for the fine mapping of qCR5/qAC5 and hold significant implications for genetic improvement of rice grain quality.关键词
水稻/垩白/直链淀粉含量/QTLKey words
rice/grain chalkiness/amylose content/QTL引用本文复制引用
云鹏,张彩娟,马廷臣,周坤能,郝栖贤,夏加发,李泽福..水稻垩白和直链淀粉含量QTL qCR5/qAC5的定位和效应验证[J].植物遗传资源学报,2025,26(11):2193-2199,7.基金项目
国家水稻产业技术体系(CARS-01-94) （CARS-01-94）
安徽省科技重大专项(2021d06050002,202103a06020008) （2021d06050002,202103a06020008）
国家重点研发计划(2021YFD1200500)The National Rice Industry Technology System(CARS-01-94) （2021YFD1200500）
The Major Scientific Technological Project of Anhui Province(2021d06050002,202103a06020008) （2021d06050002,202103a06020008）
The National Key R&D Program of China(2021YFD1200500) （2021YFD1200500）
