中国中西医结合杂志2025，Vol.45Issue(10)：1215-1221,7.DOI:10.7661/j.cjim.20250714.135
针刀调控AMPK/ULK1通路促进膝关节骨关节炎大鼠软骨细胞自噬
Needle Knife Improves Chondrocyte Autophagy in Knee Osteoarthritis Rats via Regulating AMPK/ULK1 Pathway
摘要
Abstract
Objective To observe the effects of needle knife therapy on the signaling pathway of adenosine monophosphate-activated protein kinase(AMPK)/Unc-51-like kinase 1(ULK1)in knee chondrocytes of rats with knee osteoarthritis(KOA),and to explore the potential mechanisms underlying the therapeutic effects of needle knife treatment on KOA.Methods Thirty-six SD rats were randomly divided into three groups:normal,model and needle knife group,with 12 rats in each group.The KOA rat model was established using the sodium iodoacetate(MIA)method.After modeling,the needle knife group received weekly needle knife intervention,while the normal and model groups were only grasped and fixed for 4 consecutive weeks.Pathological changes of rat chondrocytes were observed using HE staining.Mitochondrial structure and morphology were examined by electron microscopy.The levels of interleukin(IL)-1β and tumour necrosis factor(TNF)-α in cartilage tissues were detected by ELISA.The expression of autophagy proteins,including ULK1,p-ULK1,AMPK,p-AMPK,microtubule-associated protein light chain 3(LC3Ⅱ/Ⅰ),Beclin-1,and p62 were assessed by Western Blot.Additionally,mitochondrial membrane potential and reactive oxygen species(ROS)levels in cartilage tissues were measured using flow cytometry.Results Compared with the normal group,rats in the model group showed severe pathological damage in knee joint chondrocytes,a reduction in the number of autophagosomes and mitochondria,increased expression of IL-1 β,TNF-α and p62 proteins(P＜0.01),and decreased expression of ULK1,p-ULK1,AMPK,p-AMPK,LC3Ⅱ/Ⅰ and Beclin-1(P＜0.01),mitochondrial membrane potential was decreased,and ROS fluorescence intensity was enhanced(P＜0.01).In contrast,compared with the model group,the needle knife group exhibited significant improvements in pathological changes of knee joint chondrocytes,mitochondrial numbers increased,autophagosomes were slightly elevated.Expression levels of IL-1β,TNF-α,and p62 proteins were reduced(P＜0.01),expression of ULK1,p-ULK1,AMPK,p-AMPK,LC3Ⅱ/Ⅰ,and Beclin-1 protein in cartilage tissues were increased(P＜0.01),mitochondrial membrane potential was elevated,and ROS fluorescence intensity was decreased(P＜0.05,P＜0.01).Conclusions Needle knife therapy effectively ameliorates cartilage tissue damage in the knee joints of KOA model rats.The underlying mechanism may be related to the regulation of the AMPK/ULK1 signaling pathway,which enhances autophagy levels in chondrocytes of KOA rats.关键词
膝关节骨关节炎/针刀/软骨细胞自噬/AMPK/ULK1/炎性因子Key words
knee osteoarthritis/needle knife/chondrocyte autophagy/AMPK/ULK1/inflammatory factors引用本文复制引用
李蔚然,杨琪琪,周欣华,王克坡,李飞..针刀调控AMPK/ULK1通路促进膝关节骨关节炎大鼠软骨细胞自噬[J].中国中西医结合杂志,2025,45(10):1215-1221,7.基金项目
国家青年岐黄学者支持项目[No.国中医药人教函(2022)256号] （2022）
安徽省自然科学基金面上项目(No.2308085MH297) （No.2308085MH297）
安徽高校自然科学重大研究项目(No.2023AH040099) （No.2023AH040099）
安徽高校自然科学重点研究项目(No.KJ2021A0549) （No.KJ2021A0549）
