中国现代中药2025，Vol.27Issue(10)：1829-1837,9.DOI:10.13313/j.issn.1673-4890.20240830003
顺-3-己烯醛促进黄芪根培养物中黄芪皂苷合成积累作用研究
Effects of Cis-3-hexenal in Promoting Biosynthesis and Accumulation of Astragalosides in Astragalus membranaceus var.mongholicus Root Cultures
摘要
Abstract
Objective:To optimize the liquid culture system of Astragalus membranaceus var.mongholicus adventitious roots in the presence of cis-3-hexenal,establish a sample preparation method for measuring astragalosides in the culture solution and clarify the impact on the accumulation and release of astragalosides.Methods:Effects of the culture solution volume(20 and 30 mL),the inoculum size(5.0%,7.5%,and 10.0%of the culture solution volume),and the concentration of cis-3-hexenal(12.5,50.0,and 200.0 μmol·L-1)on the root growth and the content of astragalosides after four weeks of shake cultivation were examined to optimize the culture system.D101 macroporous resin was used for impurity removal,enrichment,and preparation of the culture solution samples.By using the optimized system,root cultures were processed by cis-3-hexenal for three days,and the contents of astragalosides in the root cultures and in the culture solution were dynamically determined by LC-MS/MS.Results:When the culture solution volume was 20 mL,and the inoculum size was 1.0 g,the application of 12.5 μmol·L-1 cis-3-hexenal promoted the root growth and the accumulation of astragalosides to the highest level.A column volume of 30 mL of D101 macroporous resin could completely adsorb astragalosides in 20-200 mL culture solution,and astragaloside Ⅳ,isoastragaloside Ⅰ,isoastragaloside Ⅱ,and isoastragaloside Ⅳ were mainly released into the culture solution.The treatment with 12.5 μmol·L-1 cis-3-hexenal for 4-8 h facilitated the most effective accumulation of astragaloside Ⅰ and cycloastragenoside Ⅱ.Conclusion:When the culture solution volume was 20 mL,adding 12.5 μmol·L-1 cis-3-hexenal to the liquid culture system of Astragalus membranaceus var.mongholicus adventitious roots is beneficial to the growth of the root cultures and the accumulation of astragalosides.关键词
蒙古黄芪/不定根培养/顺-3-己烯醛/黄芪皂苷Key words
Astragalus membranaceus(Fisch)Bge.var.mongholicus(Bge)Hsiao/adventitious root culture/cis-3-hexenal/astragaloside分类
中医学引用本文复制引用
高征豪,胡海运,贾金萍,孙海峰,柴智..顺-3-己烯醛促进黄芪根培养物中黄芪皂苷合成积累作用研究[J].中国现代中药,2025,27(10):1829-1837,9.基金项目
山西省自然科学基金面上项目(202103021224017) （202103021224017）
山西省黄芪资源产业化及产业国际化协同创新中心项目(HQXTCXZX2016-002,HQXTCXZX2016-003,HQXTCXZX2016-016) （HQXTCXZX2016-002,HQXTCXZX2016-003,HQXTCXZX2016-016）
