中国现代中药2025，Vol.27Issue(10)：1847-1853,7.DOI:10.13313/j.issn.1673-4890.20240905002
黄芪须根总皂苷提取工艺优化
Optimization of Extraction Process of Total Saponins from Astragalus membranaceus Fibrous Roots
摘要
Abstract
Objective:To optimize the ultrasonic-assisted extraction process of total saponins from Astragalus membranaceus fibrous roots using response surface methodology.Methods:The effects of ethanol volume fraction,solid-to-liquid ratio,extraction time,and extraction temperature on the extraction yield of total saponins from A.membranaceus fibrous roots were investigated through single-factor experiments.Based on the single-factor results,key factors affecting the extraction yield of total saponins from A.membranaceus fibrous roots were identified,and their optimal value ranges were determined.Using ethanol volume fraction,solid-to-liquid ratio,extraction time,and extraction temperature as independent variables,a four-factor,three-level optimization experiment was designed using response surface methodology.It was then employed to optimize the extraction process.Results:The single-factor experiments indicated that the optimal extraction conditions were an ethanol volume fraction of 60%,a solid-to-liquid ratio of 1:16,an ultrasonic extraction time of 25 min,and an extraction temperature of 60℃.Regression model analysis showed that the optimal extraction conditions for the extraction yield of total saponins from A.membranaceus fibrous roots were an ethanol volume fraction of 60%,a solid-to-liquid ratio of 1:16,an ultrasonic extraction time of 25 min,and an extraction temperature of 57℃.Under these conditions,the extraction yield of total saponins from A.membranaceus fibrous roots reached 2.82%.Conclusion:An efficient extraction technology for total saponins from A.membranaceus fibrous roots was established,providing a reference for the high-efficiency utilization and development of A.membranaceus resources.关键词
黄芪须根/超声波提取/总皂苷/响应面优化Key words
Astragalus membranaceus fibrous roots/ultrasonic extraction/total saponins/response surface optimization分类
医药卫生引用本文复制引用
崔丽艳,杨佼丽,韩杰宏,苑英欣,罗伟栋,王德富,牛颜冰..黄芪须根总皂苷提取工艺优化[J].中国现代中药,2025,27(10):1847-1853,7.基金项目
财政部和农业农村部:国家现代农业产业技术体系资助项目 （）
山西农业大学"引进人才科研启动工程"项目(2023BQ132) （2023BQ132）
恒山黄芪产业研究院项目(XDHZHQY2022-03) （XDHZHQY2022-03）
山西省博士毕业生来晋工作科研项目(SXBYKY2024026) （SXBYKY2024026）
