中国现代中药2025，Vol.27Issue(10)：1859-1869,11.DOI:10.13313/j.issn.1673-4890.20250319002
人参内生草假单胞菌nH121及其糖苷酶对天然人参皂苷的转化活性研究
Transformation Activity of Ginsenosides by Endophytic Pseudomonas poae nH121 and Its Glycosidase from Panax ginseng
摘要
Abstract
Objective:To prepare rare ginsenosides via the biotransformation of natural ginsenosides and investigate the transformation mechanisms.Methods:Endophytic bacteria with ginsenoside-transforming activity were screened from the roots of two-year-old Panax ginseng.After preliminary identification by 16S rRNA sequencing,the strain nH121 with the highest activity was further characterized,and its hydrolytic activity on individual ginsenosides was verified.The β-glucosidase nH-pp-bgIX was cloned and expressed in vitro,and its catalytic properties were studied.Bioinformatics analysis and molecular docking were performed.Results:Thirty-eight strains of endophytic bacteria were isolated,primarily belonging to Pseudomonas spp.,followed by Paenibacillus spp.,Bacillus spp.,and Serratia spp.The strain nH121,identified as Pseudomonas poae,exhibited transformation activity on ginsenosides Rb1,Rd,Rg1,and Re.Catalytic experiments revealed that the β-glucosidase of this strain could sequentially hydrolyze the glucopyranosyl moiety at the C20 position of the protopanaxadiol-type ginsenoside Rb1,following the pathway Rb1→Rd→Rg3.Bioinformatics analysis indicated that the enzyme belonged to the glucoside hydrolase 3 family,and molecular docking identified its key catalytic residues.Conclusion:This study successfully isolated Pseudomonas poae nH121 with ginsenoside transformation activity,achieved the in vitro functional verification of its β-glucosidase,and elucidated the catalytic mechanism.These results provide microbial resources and experimental evidence for the biotransformation of rare ginsenosides.关键词
生物转化/稀有人参皂苷/草假单胞菌/β-葡萄糖苷酶/分子对接Key words
biotransformation/rare ginsenosides/Pseudomonas poae/β-glycosidase/molecular docking分类
医药卫生引用本文复制引用
耿鑫,赵金彤,于洪颖,甘少娟,杨成民,张争..人参内生草假单胞菌nH121及其糖苷酶对天然人参皂苷的转化活性研究[J].中国现代中药,2025,27(10):1859-1869,11.基金项目
国家重点研发计划项目(2022YFC3501502) （2022YFC3501502）
中国医学科学院医学与健康科技创新工程项目(2022-I2M-1-017) （2022-I2M-1-017）
