中国现代中药2025，Vol.27Issue(10)：1870-1880,11.DOI:10.13313/j.issn.1673-4890.20250410004
菊苣OSC基因家族分析及环阿屯醇合酶的鉴定
Analysis of the OSC Gene Family in Cichorium intybus and Identification of A Cycloartenol Synthase
摘要
Abstract
Objective:To identify and perform a bioinformatic analysis of the 2,3-oxidosqualene cyclase(OSC)gene family in Cichorium intybus L.and clone and functionally characterize the cycloartenol synthase gene.Methods:The whole-genome data of C.intybus were used for homologous sequence alignment and domain screening,and thus members of the OSC gene family were subsequently subjected to bioinformatics analysis.The phylogenetic analysis and key functional site prediction were used to identify the putative cycloartenol synthase of C.intybus.The cycloartenol synthase gene was then cloned by polymerase chain reaction,and its function was verified by detecting the transient expression product in tobacco via gas chromatography-mass spectrometry.Results:Twelve members of the OSC gene family were identified in C.intybus,distributed across six chromosomes.The encoded proteins contained 696-799 amino acid residues and all possessed the conserved domain of the PLN03012 superfamily.All the members exhibited response elements for various plant hormones,abiotic stresses,and growth and development.The phylogenetic analysis divided the OSC gene family of C.intybus into two subfamilies.The cycloartenol synthase of C.intybus was identified and named CiOSC3,which was verified to exhibit the cycloartenol synthase activity.Conclusion:This study presents the first systematic identification and analysis of the OSC gene family in C.intybus and successfully identifies a functional cycloartenol synthase,CiOSC3.关键词
菊苣/2,3-氧化鲨烯环化酶/生物信息学分析/环阿屯醇合酶Key words
Cichorium intybus L./2,3-oxysqualene cyclase(OSC)/bioinformatics analysis/cycloartenol synthase分类
中医学引用本文复制引用
徐艳茹,张彬,王晨晨,冯清香,朱哈修,徐朝阳,刘雪,李大勇,唐金富,周军..菊苣OSC基因家族分析及环阿屯醇合酶的鉴定[J].中国现代中药,2025,27(10):1870-1880,11.基金项目
北京市农林科学院创新能力建设专项(KJCX20251008) （KJCX20251008）
北京市农林科学院蔬菜研究所改革与发展项目(KYCX202404). （KYCX202404）
