中国现代中药2025，Vol.27Issue(10)：1898-1905,8.DOI:10.13313/j.issn.1673-4890.20241030001
山西不同种植模式蒙古黄芪性状鉴别和荧光显微特征的数字化表征
Digital Characterization of Trait Identification and Fluorescence Microscopic Features of Astragalus membranaceus var.mongholicus under Different Planting Patterns in Shanxi Province
摘要
Abstract
Objective:To investigate the macroscopic traits and microscopic characteristics of Astragalus membranaceus(Fisch.)Bge.var.mongholicus(Bge.)Hsiao under different cultivation patterns and establish an identification method based on digital characterization.Methods:The surface and cross-sectional structures of the samples were observed using stereo fluorescence microscopy,optical microscopy,and a full-field high-resolution fluorescence live-cell imaging system under visible light,red fluorescence(TexRE),green fluorescence(EGFP),and ultraviolet light(DAPI)modes.Digital analysis of the structural features was performed based on color and morphology.Result:Wild-simulated direct-seeded and transplanted A.membranaceus var.mongholicus exhibited differences in external morphology.Their cross-sectional digital profiles were also distinct.Furthermore,stained samples revealed clear differences in the degree of lignification and starch content when observed under optical microscopy.Conclusion:Through multiple comparative analyses,methods for differentiating A.membranaceus var.mongholicus from different planting patterns based on macroscopic traits and microscopic features were established,providing a scientific basis and methodological framework for further research on this species.关键词
蒙古黄芪/仿野生直播/移栽/性状鉴别/荧光显微特征/全视野高分辨荧光活细胞工作站/染色Key words
Astragalus membranaceus(Fisch.)Bge.var.mongholicus(Bge.)Hsiao/wild-simulated direct-seeded/transplanted/trait identification/fluorescence microscopic feature/full-field high-resolution fluorescence live-cell imaging system/staining分类
中医学引用本文复制引用
赵瑛瑛,曹慧敏,武开智,杨丽婷,赵晓琴,康帅..山西不同种植模式蒙古黄芪性状鉴别和荧光显微特征的数字化表征[J].中国现代中药,2025,27(10):1898-1905,8.基金项目
大同市重点研发计划项目(2022022) （2022022）
