中国现代中药2025，Vol.27Issue(10)：1947-1952,6.DOI:10.13313/j.issn.1673-4890.20241206001
基于UPLC的冬葵子配方颗粒特征图谱建立及咖啡酸含量测定
Establishment of Characteristic Chromatogram of Malva Seed Formula Granules and Content Determination of Caffeic Acid Based on UPLC
蒋春亮 1姚杭琦 1程立伟 1周永康 1张志强 1吉艳慧1
作者信息
- 1. 北京康仁堂药业有限公司,北京 101301||中药配方颗粒关键技术国家地方联合工程研究中心,天津 301700||北京市中药配方颗粒工程技术研究中心,北京 101301
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摘要
Abstract
Objective:To establish a characteristic chromatogram for malva seed formula granules using ultra-high performance liquid chromatography(UPLC)and determine the mass fraction of caffeic acid.Methods:Waters UPLC® BEH Shield RP18 column(100 mm×2.1 mm,1.7 μm)was used,with acetonitrile and 0.1%phosphoric acid aqueous solution as mobile phase for gradient elution.Column temperature was 30℃;detection wavelength was 322 nm;flow rate was 0.30 mL·min-1;sample volume was 3 μL.Based on the above chromatographic conditions,a characteristic chromatogram of the malva seed formula granules was established.By using the chromatographic peak of caffeic acid as the reference(S)peak,the similarity between the characteristic chromatogram of the standard decoction of malva seed and the characteristic chromatogram of malva seed granules was evaluated.Moreover,a method for determining the content of caffeic acid in malva seed formula granules was established.Results:A total of eight characteristic peaks were identified in the malva seed formula granules'characteristic chromatogram,with peak 2 corresponding to caffeic acid.The similarity between the characteristic chromatogram of eight batches of malva seed formula granules and the control characteristic chromatogram was greater than 0.950.The mass fraction of caffeic acid in eight batches of malva seed formula granules ranged from 0.128 to 0.304 mg·g-1.Conclusion:The established characteristic chromatogram and content determination method for malva seed formula granules are simple,with good repeatability and high accuracy,and they provide reference for effectively controlling the quality of malva seed formula granules.关键词
冬葵子/咖啡酸/配方颗粒/含量测定/特征图谱Key words
malva seed/caffeic acid/formula granules/content determination/characteristic chromatogram分类
中医学引用本文复制引用
蒋春亮,姚杭琦,程立伟,周永康,张志强,吉艳慧..基于UPLC的冬葵子配方颗粒特征图谱建立及咖啡酸含量测定[J].中国现代中药,2025,27(10):1947-1952,6.
