中国现代中药2025，Vol.27Issue(10)：1953-1959,7.DOI:10.13313/j.issn.1673-4890.20250124004
鹿血晶电子束辐照灭菌的安全性评价方法
Safety Assessment of Electron Beam Irradiation for Deer Blood Crystals
摘要
Abstract
Objective:To evaluate the safety of deer blood crystals sterilized by electron beam irradiation.Methods:Deer blood crystals were Deer blood crystals were subjected to irradiation at doses of 4,6,8,10,20,30,and 40 kGy using an electron linear accelerator.The changes of microbial load of deer blood crystals before and after irradiation were compared.Genetic damage in the irradiated samples was assessed using a gene testing method.Zebrafish embryos were used as the research objects to investigate the toxicity of deer blood crystals irradiated by electron beam.Results:When the electron beam irradiation dose reached 8 kGy,the microbial load of deer blood crystals decreased significantly.When the electron beam irradiation doses reached 10,20,30,and 40 kGy,no obvious damage appeared in the deer-specific genes of deer blood crystals.When the electron beam irradiation doses reached 10,20,30 and 40 kGy,deer blood crystals did not exert obvious toxicity in zebrafish embryos.Conclusion:The electron beam irradiation at a dose of 8 kGy demonstrated good sterilization effect,and no significant genetic damage or toxicity appeared in deer blood crystals after high-dose irradiation.关键词
鹿血晶/电子束辐照/60Co-γ射线/灭菌/基因检测/斑马鱼Key words
deer blood crystal/electron beam irradiation/60Co-γ ray/sterilization/genetic testing/zebrafish分类
中医学引用本文复制引用
孙波,吴嘉琪,焦娇娇,吴艳娥,裴庆秀,樊永丽,程燕,何明芳,濮存海..鹿血晶电子束辐照灭菌的安全性评价方法[J].中国现代中药,2025,27(10):1953-1959,7.基金项目
国家自然科学基金项目(82272671,82203219) （82272671,82203219）
江苏省科技项目(BK20210546) （BK20210546）
