中药药理与临床2025，Vol.41Issue(9)：15-20,6.
基于GLUT1/HK2代谢轴探讨沙参麦冬汤含药血清对人非小细胞肺癌A549细胞增殖、侵袭、迁移和凋亡的影响
Effects of Shashen(沙参)Maidong(麦冬)Decoction-Containing Serum on Proliferation,Invasion,Migration,and Apoptosis of Human Non-Small Cell Lung Cancer A549 Cells Based on GLUT1/HK2 Metabolic Axis
摘要
Abstract
Objective:To observe the effect of Shashen(沙参)Maidong(麦冬)Decoction-containing serum on the proliferation,apoptosis,migra-tion,and invasion of human non-small cell lung cancer A549 cells and explore its possible mechanism.Methods:The Shashen Maidong De-coction-containing serum was prepared,and the human non-small cell lung cancer A549 cell line was cultured in vitro.In addition,the optimal concentration and time of action of the Shashen Maidong Decoction-containing serum were screened by cell proliferation and activity assay(CCK-8)for subsequent experiments.A549 cells were divided into a blank group,a model control group,and Shashen Maidong Decoction-containing serum groups.The cloning ability,migration,and invasion ability of each group were tested by plate cloning test,cell scratch test,and cell invasion test,respectively.Flow cytometry was used to detect the effect of Shashen Maidong Decoction-containing serum on A549 cell apoptosis.Western blot was used to detect the effect of the Shashen Maidong Decoction-containing serum on the protein expressions of glucose transporter type 1(GLUT1),hexokinase II(HK2),lactate dehydrogenase A(LDHA)in A549 cells.A glucose test kit and lactic acid test kit were used to test the effect on glucose consumption and lactic acid production of A549 cells.Results:Compared with that in the blank group and the model control group,the absorbance in the middle-dose and high-dose groups of Shashen Maidong Decoction was decreased sig-nificantly(P<0.01),indicating that the middle-dose and high-dose groups of Shashen Maidong Decoction could significantly inhibit the pro-liferation of A549 cells.Compared with those in the blank group and model control group,the cloning ability and migration ability of A549 cells in the Shashen Maidong Decoction groups were decreased(P<0.01),and the invasion ability was reduced significantly(P<0.01),while the total apoptosis rate was increased significantly(P<0.01).Compared with those in the blank group and model control group,the protein and mRNA expressions of GLUT1,HK2,and LDHA in the Shashen Maidong Decoction groups were significantly down-regulated(P<0.01).Conclusion:Shashen Maidong Decoction can significantly inhibit the malignant biological behavior of human non-small cell lung cancer A549 cells,and its mechanism may be related to inhibiting the GLUT1/HK2 metabolic axis,down-regulating the expression of glycolyt-ic-related enzymes,inhibiting the aerobic glycolysis of A549 cells,and promoting cell apoptosis.关键词
沙参麦冬汤含药血清/非小细胞肺癌/增殖/侵袭/迁移Key words
Shashen(沙参)Maidong(麦冬)Decoction-containing serum/Non-small cell lung cancer/Proliferation/Invasion/Migration引用本文复制引用
刘亚琪,郭明星,甘盼盼,全毅红..基于GLUT1/HK2代谢轴探讨沙参麦冬汤含药血清对人非小细胞肺癌A549细胞增殖、侵袭、迁移和凋亡的影响[J].中药药理与临床,2025,41(9):15-20,6.基金项目
武汉市卫生健康科研基金资助项目(编号:WZ24Q01). （编号:WZ24Q01）
