中药药理与临床2025，Vol.41Issue(9)：27-32,6.
基于转录组学和"叠加分析"原理拆方分析化癥散积方抗肝癌作用机制的研究
Transcriptomics and Superposition Analysis Based on Formula Dismantling Reveal the Mechanism of Anti-hepatocellular Carcinoma Effect of Huazheng Sanji(化癥散积)Formula
摘要
Abstract
Objective:To analyze the differential genes between single Chinese herbal medicines and the compound formula of Huazheng Sanji(化癥散积)Formula based on transcriptomics and superposition analysis and explore the compatibility mechanism of this formula.Methods:Based on the transcriptomics data,the superposition analysis was adopted to analyze the differential genes of the anti-hepatocellular carcinoma effect between Huazheng Sanji Formula and 11 Chinese herbal medicines consisting this formula.The new efficacy genes and potentiating genes of Huazheng Sanji Formula compared with single Chinese herbal medicines after decocting were identified.Results:Compared with sin-gle Chinese herbal medicines,Huazheng Sanji Formula showed one potentiating gene encoding the TNF superfamily member 10(Tnfsf10)and two new efficacy genes encoding carbonic anhydrase 3(Ca3)and mucin 15(Muc15).Conclusion:Huazheng Sanji Formula produces a po-tentiating gene(Tnfsf10)and two new anticancer genes(Ca3 and Muc15)after decocting.The compound formula enhances the original function after Junchenzuoshi(君臣佐使)compatibility and decocting and produces new functions to achieve enhanced anticancer effect,which provides a scientific basis for the anti-cancer treatment guided by the theory of Zangdufuhui(脏毒腑秽).关键词
原发性肝癌/叠加分析/化癥散积方/转录组学Key words
Primary hepatocellular carcinoma/Superposition analysis/Huazheng Sanji(化癥散积)Decoction/Transcriptomics引用本文复制引用
孟香彤,刘扬扬,王松,苏博扬,刘志远,冷炎,刘铁军,熊壮..基于转录组学和"叠加分析"原理拆方分析化癥散积方抗肝癌作用机制的研究[J].中药药理与临床,2025,41(9):27-32,6.基金项目
国家自然科学基金资助项目(编号:81804007) （编号:81804007）
吉林省自然科学基金资助项目(编号:20200201590JC) （编号:20200201590JC）
吉林省教育厅"十三五"科学技术资助项目(编号:JJKH20200885KJ). （编号:JJKH20200885KJ）
