中药药理与临床2025，Vol.41Issue(9)：59-64,6.
羟基红花黄色素A通过PI3K/AKT/FOXO1通路减轻LPS诱导的神经炎症
Reduction of LPS-Induced Neuroinflammation by Hydroxysafflor Yellow Pigment A via PI3K/AKT/Foxo1 Signaling Pathway
摘要
Abstract
Objective:To investigate the role and mechanism of hydroxylsafflower yellow pigment A in lipopolysaccharide(LPS)-induced inflam-mation responses in BV2 cells and LPS-induced neuroinflammation in mice.Methods:The BV2 cell inflammation models were induced by LPSs in vitro.The models were divided into blank control group,model control group,2 μmol/L dexamethasone group,5 μmol/L hydroxylsaf-flower yellow pigment A group,and 10 μmol/L hydroxylsafflower yellow pigment A group.BV2 cell viability was detected by CCK8.The con-tents of interleukin-6(IL-6)and tumor necrosis factor α(TNF-α)in the supernatant were detected by ELISA.The NO content in cell super-natant was detected by Griess method.In vivo experiments randomly divided mice into normal control group,model control group,20 mg/kg aspirin enteric-coated tablet group,5 mg/kg hydroxylsafflower yellow pigment A group,and 10 mg/kg hydroxylsafflower yellow pigment A group.The anxiety-like behavior and cognitive dysfunction of mice were observed by open field test and new object recognition test.HE stai-ning was employed to observe the histopathological changes in CA1,CA3,and cortex regions of the hippocampus.Serum contents of IL-6 and TNF-α were detected by ELISA.Western blot was used to detect the PI3K/Akt/FOXO1 signaling pathway-related protein expression in the mouse cortex.Results:In LPS-induced BV2 cell inflammation model,CCK8 results showed that 5,10,and 20 μmol/L hydroxylsafflower yellow pigment A were the safe dose for LPS-induced BV2 cell inflammation responses(P>0.05).Compared with those of the blank control group,the contents of IL-6,TNF-α,and NO in cell supernatant of the model control group were significantly increased(P<0.01).Compared with those of the model control group,the contents of IL-6,TNF-α,and NO in cell supernatant of the 5 and 10 μmol/L hydroxylsafflower yellow pigment A groups were significantly decreased(P<0.05 or P<0.01).In the LPS-induced mouse neuroinflammation model,the anxie-ty-like behavior and cognitive dysfunction of mice were more significant in the open field test and the new object recognition test in the model control group(P<0.01).The arrangement of neurons in CA1,CA3,and cortex regions of the hippocampus of mice was disordered,and karyopyknosis appeared.The serum contents of IL-6 and TNF-α were significantly increased(P<0.01).The protein expressions of PI3K,p-Akt,and p-FoxO1 were significantly decreased(P<0.01).Compared with those in the model control group,the anxiety-like behavior and cognitive dysfunction of mice in the 5 and 10 mg/kg hydroxylsafflower yellow pigment A groups in the open field test and the new object recog-nition test were significantly alleviated(P<0.05 or P<0.01).The neurons in CA1,CA3,and cortex regions of the hippocampus of mice were arranged neatly and structurally intact.The serum contents of IL-6 and TNF-α were significantly decreased(P<0.01).The protein expres-sions of PI3K,p-Akt,and p-FOXO1 were significantly increased(P<0.05 or P<0.01).Conclusion:Hydroxylsafflower yellow pigment A can alleviate neuroinflammation and cognitive dysfunction induced by LPSs,and its mechanism may be related to the regulation of the PI3K/Akt/FOXO1 signaling pathway.关键词
羟基红花黄色素A/脂多糖(LPS)/神经炎症/磷脂酰肌醇-3激酶/蛋白激酶/FOXO1信号通路Key words
Hydroxylsafflower yellow pigment A/Lipopolysaccharide(LPS)/Neuroinflammation/PI3K/Akt/FOXO1 signaling pathway引用本文复制引用
冯永岗,韩云,王咪咪,单凯欣,范国旗,苗明三,方晓艳..羟基红花黄色素A通过PI3K/AKT/FOXO1通路减轻LPS诱导的神经炎症[J].中药药理与临床,2025,41(9):59-64,6.基金项目
河南省中医药科学研究专项(编号:20-21ZY2156) （编号:20-21ZY2156）
河南省科技研发计划联合基金(编号:222301420091). （编号:222301420091）
