中药药理与临床2025，Vol.41Issue(10)：11-16,6.
心脑络泰胶囊通过调控Toll样受体信号通路缓解大鼠心肌梗死作用机制研究
Mechanism of Xinnao Luotai Capsules in Alleviating Myocardial Infarction in Rats by Regulating the Toll-Like Receptor Signaling Pathway
摘要
Abstract
Objective:To explore the mechanism of Xinnao Luotai(心脑络泰)Capsules on myocardial infarction(MI)via the Toll-like receptor(TLR)signaling pathway.Methods:One hundred male SD rats were randomly divided into a sham operation control group,a model control group,a Naoxintong(脑心通)(110 mg/kg)group,and Xinnao Luotai Capsules groups(64.8,32.4,16.2 mg/kg).An MI model was es-tablished by ligating the left anterior descending coronary artery.Syndromes of the model were manifested by tongue characteristics,exhaustive swimming time,and grip strength.MI rate was measured using triphenyl tetrazolium chloride(TTC)staining.The pharmacodynamic efficacy of these medicines was observed by electrocardiogram,myocardial enzymes,and serum inflammatory factors.Pathological changes of myocardi-um tissue were observed by hematoxylin and eosin(HE)staining,while Tlr-related mRNA and protein expression levels were detected by qRT-PCR and Western blot.Results:Compared with the sham operation control group,the model group exhibited significant ST segment ele-vation and massive infarct areas,confirming successful modeling.Tongue characteristics,exhaustive swimming time,and grip strength sugges-ted that the MI model was induced by Qixu Xueyu(气虚血瘀)syndrome.Myocardial enzyme levels and inflammatory factors were signifi-cantly elevated in model rats(P<0.01).Pathological analysis revealed local speckled infarction and dissolution in myocardial cells,with a large number of inflammatory cell infiltrations.The protein and mRNA expressions of interferon-α(IFN-α),interferon regulatory factor 7(IRF7),myeloid differentiation factor 88(MyD88),nuclear factor κB(NF-κB),Toll-like receptors(TLR2,TLR4,TLR7,and TLR9),and tumor necrosis factor-α(TNF-α)were significantly increased in myocardial tissue(P<0.05 or P<0.01).Compared with the model control group,all Xinnao Luotai groups showed reduced myocardial enzymes and inflammatory factors,while the pathological damage was recovered.The protein and mRNA expressions of IFN-α,IRF7,MyD88,NF-κB,TLR2,TLR4,TLR7,TLR9,and TNF-α were decreased in myocardial tis-sue,while the most significant interventive effect occurred in the 64.8 mg/kg Xinnao Luotai group(P<0.05 or P<0.01).Conclusion:By regulating the TLR related signaling pathway,Xinnao Luotai Capsules can inhibit cardiac inflammation and alleviate MI with Qixu Xueyu syn-drome,improving myocardial tissue.关键词
心脑络泰胶囊/心肌梗死/Toll样受体/免疫和炎症反应Key words
Xinnao Luotai Capsules/Myocardial infarction/Toll-like receptor/Immune and inflammatory response引用本文复制引用
张振东,王鹏,刘秋月,王克婧,刘树民,闫久江,孟杰,卢芳,陈平平..心脑络泰胶囊通过调控Toll样受体信号通路缓解大鼠心肌梗死作用机制研究[J].中药药理与临床,2025,41(10):11-16,6.基金项目
黑龙江省科技厅药企合作课题(编号:2023-Z004). （编号:2023-Z004）
