中药药理与临床2025，Vol.41Issue(10)：32-37,6.
基于血清代谢组学探讨克银丸对血热证银屑病模型大鼠的干预机制
Intervention Mechanism of Keyin Pills on Psoriasis Model Rats with Blood Heat Syndrome Based on Serum Metabolomics
摘要
Abstract
Objective:To investigate the effect of Keyin(克银)Pills on serum metabolism of psoriasis model rats with blood heat syndrome by metabolomics,and to reveal the internal mechanism of inflammatory response improvement in rats.Methods:A psoriasis rat model with blood heat syndrome was established.After the rats were administered Keyin Pills by gavage for 4 weeks,the following aspects were observed:the body weight,food intake,water intake,and rectal temperature,pathological changes in the dorsal skin by Hematoxylin-eosin(HE)staining,tumor necrosis factor-α(TNF-α),interleukin(IL)-1β,IL-17A,IL-22,IL-23 proteins,and mRNA expression levels in the skin lesions by Western blot and quantitative real-time reverse transcription-polymerase chain reaction(qRT-PCR),as well as serum metabolomics analysis by ultraperformance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS).All these aim to identify potential biomarkers and related metabolic pathways affected by Keyin Pills.Results:Compared with the normal control group,the TNF-α,IL-1β,IL-17A,IL-22,IL-23 protein,and mRNA expression were significantly upregulated in the model control group(P<0.05 or P<0.01).Compared with the model control group,the skin lesions were effectively alleviated in the Keyin Pill administration group,with the TNF-α,IL-1β,IL-17A,IL-22,IL-23 protein,and mRNA expression levels being significantly downregulated in skin lesions(P<0.05 or P<0.01).Combined with multivariate statistics and online databases,a total of 22 differential metabolites were screened out in the normal con-trol group,model control group,and Keyin Pill administration group.The metabolites were mainly enriched in multiple pathways,including sphingolipid metabolism and tryptophan metabolism.Conclusion:Keyin Pills exert significant anti-inflammatory and skin barrier repair effects in psoriasis rats with blood heat syndrome by regulating the sphingolipid metabolism and tryptophan metabolism pathways.关键词
克银丸/银屑病/血热证/炎症/血清代谢组学/鞘脂代谢通路/色氨酸代谢通路Key words
Keyin Pills/Psoriasis/Blood heat syndrome/Inflammation/Serum metabolomics/Sphingolipid metabolic pathway/Tryptophan met-abolic pathway引用本文复制引用
黄琳,孙慧娟,邓戈宇,王宇,高蕊,张萌萌,张振东,刘树民..基于血清代谢组学探讨克银丸对血热证银屑病模型大鼠的干预机制[J].中药药理与临床,2025,41(10):32-37,6.基金项目
国家重点研发计划-中医药现代化(编号:2022YFC3502100、2022YFC3502102、2022YFC3502102-04). （编号:2022YFC3502100、2022YFC3502102、2022YFC3502102-04）
