中药药理与临床2025，Vol.41Issue(10)：57-61,5.
欧前胡素通过SHP1/PDGFRβ通路抑制肝星状细胞活化的实验研究
Imperatorin Inhibits Hepatic Stellate Cell Activation via the SHP1/PDGFRβ Pathway
摘要
Abstract
Objective:To explore the effect of imperatorin on the activation of human hepatic stellate cells(LX-2)and the possible mechanism.Methods:LX-2 cells were randomly grouped as follows:transforming growth factor-β1(TGF-β1),imperatorin(10,20,and 40 μmol/L),and silybin(5 μmol/L).After drug intervention,the activation and proliferation of LX-2 cells were measured by the CCK-8 assay.Cell migration was assessed via a scratch assay.The levels of interleukin-6(IL-6)and type I collagen(Col-I)in the cell supernatant were determined by ELISA.The protein levels of α-smooth muscle actin(α-SMA),Src homology domain 2-containing protein tyrosine phosphatase 1(SHP1),and platelet-derived growth factor receptor β(PDGFRβ),as well as the phosphorylation levels of Janus kinase 1(JAK1)and signal trans-ducer and activator of transcription 3(STAT3),were determined by Western blot.Results:Compared with those in the normal control group,the LX-2 cells in the model control group exhibited a typical fibroblast-like morphological change with a long spindle shape,increased prolifer-ation and migration(P<0.01),downregulated protein level of SHP1(P<0.01),and upregulated protein levels of α-SMA,PDGFRβ,IL-6,and Col-I,as well as the phosphorylation levels of JAK1 and STAT3(P<0.05 or P<0.01).In comparison with the model control group,in-tervention with imperatorin improved the morphology of LX-2 cells,decreased the cell proliferation and migration(P<0.05 or P<0.01),up-regulated the protein level of SHP1(P<0.05),and down-regulated the protein levels of α-SMA,PDGFRβ,IL-6,and Col-I and the phospho-rylation levels of JAK1 and STAT3(P<0.05 or P<0.01).Conclusion:Imperatorin can inhibit the activation of LX-2 cells by regulating the SHP1/PDGFRβ pathway.关键词
欧前胡素/肝纤维化/肝星状细胞/含SH2结构域的蛋白酪氨酸磷酸酶1/血小板衍生的生长因子受体β通路Key words
Imperatorin/Hepatic fibrosis/Hepatic stellate cell/Src-homology domain 2-containing protein tyrosine phosphatase1/platelet-de-rived growth factor receptor β pathway引用本文复制引用
陈志惠,罗文川,陈子馨,陈亚萍,王鹭琦,南丽红,黄枚..欧前胡素通过SHP1/PDGFRβ通路抑制肝星状细胞活化的实验研究[J].中药药理与临床,2025,41(10):57-61,5.基金项目
福建中医药大学高层次人才科研启动资金项目(编号:X2019007-人才) （编号:X2019007-人才）
福建中医药大学基础提升项目(编号:XJC2022003) （编号:XJC2022003）
福建省自然科学基金项目(编号:2021J01926) （编号:2021J01926）
福建省卫健委科技计划项目(编号:2021zyyj67). （编号:2021zyyj67）
