中医药信息2025，Vol.42Issue(10)：12-16,5.DOI:10.19656/j.cnki.1002-2406.20251003
接骨柔筋方促进骨折后成血管及改善骨代谢的作用机制研究
Mechanism Study of Jiegu Roujin Formula in Promoting Angiogenesis and Improving Bone Metabolism after Fracture
摘要
Abstract
Objective:To evaluate the drug safety of Jiegu Roujin Formula and explore its mechanism in promoting angiogenesis and improving bone metabolism in rats with tibial fracture.Methods:SD rats were used to establish tibial fracture models.After successful modeling,rats were randomly divided into model group,Jiegu Roujin Formula group,and Xianling Gubao group(15 rats each),with an additional 15 rats as blank group.The blank group received no intervention;the model group received saline[10 mg/(kg·d)]by gavage;the Xianling Gubao group received Xianling Gubao suspension[10 mg/(kg·d)]by gavage;and the Jiegu Roujin Formula group received the formula[13.5 mg/(kg·d)]by gavage,all administered twice daily for 2 weeks.Drug safety was assessed by serum creatinine and urea nitrogen levels;Bone metabolism was evaluated by alkaline phosphatase,calcium,phosphorus,and testosterone levels;Hemorheological changes were measured by fibrinogen,erythrocyte sedimentation rate,plasma viscosity,and hematocrit after the Jiegu Roujin Formula intervention;Angiogenesis was assessed by CD31 immunofluorescence staining of microvessel density in fracture site soft tissues.Results:Compared with the model group,both Xianling Gubao and Jiegu Roujin Formula groups showed decreased creatinine and urea nitrogen levels(P<0.05),increased alkaline phosphatase,calcium,and phosphorus levels(P<0.05),significantly reduced erythrocyte sedimentation rate,plasma viscosity,and hematocrit(P<0.05),and increased CD31-positive microvessel proportion in gastrocnemius and soleus muscles at the fracture site(P<0.05).The Jiegu Roujin Formula group additionally demonstrated significantly lower testosterone and fibrinogen levels(P<0.05),although the there are descending trend for testosterone level and fibrinogen in Xianling Gubao group,the difference was not statistically significant(P>0.05).Conclusion:Jiegu Roujin Formula demonstrates good safety and effects in promoting angiogenesis,improving hemorheology,and enhancing bone metabolism.Its fracture-healing mechanism may involve angiogenesis promotion and bone metabolism regulation.关键词
骨折/筋骨并重/接骨柔筋方/骨代谢/成血管Key words
Fracture/Simultaneous bone-tendon treatment/Jiegu Roujin Formula/Bone metabolism/Angiogenesis引用本文复制引用
张辰,李玙璠,徐世红,王培鑫,朱欢,赵军..接骨柔筋方促进骨折后成血管及改善骨代谢的作用机制研究[J].中医药信息,2025,42(10):12-16,5.基金项目
甘肃省科技计划项目(21JR7RA681,22YF7FA104,24YFFA065) （21JR7RA681,22YF7FA104,24YFFA065）
兰州市科技计划项目(2023-2-95) （2023-2-95）
甘肃中医药大学成果转化项目(2023CGZH-3) （2023CGZH-3）
甘肃中医药大学研究生创新创业基金项目(2025CXCY-026) （2025CXCY-026）
