中医药信息2025，Vol.42Issue(10)：17-22,6.DOI:10.19656/j.cnki.1002-2406.20251004
电针会阳、中髎穴对压力性尿失禁大鼠尿道横纹肌细胞的影响
Effect of Electroacupuncture at Huiyang(BL35)and Zhongliao(BL33)Acupoints on Striated Muscle Cells of the Urethra in Rats with Stress Urinary Incontinence
摘要
Abstract
Objective:To observe the effect of electroacupuncture stimulation at Huiyang(BL35)and Zhongliao(BL33)acupoints on urethral striated muscle cells in ovariectomized rat models of stress urinary incontinence(SUI).Methods:Twenty-three female SD rats were randomly divided into a blank group(6 rats)and a model establishment group(17 rats).The model establishment group underwent ovariectomy to create SUI model rats.A urine sneeze test was conducted,and 12 successfully modeled SD rats were selected and randomly divided into two groups:model group and electroacupuncture group,with 6 rats in each group.The electroacupuncture group received electroacupuncture stimulation at Zhongliao(BL33)and Huiyang(BL35)acupoints.The blank group and the model group received sham needle stimulation at the same acupoints.After seven consecutive interventions,a urine sneeze test was performed,and all rats were euthanized.Urethral striated muscle tissue samples were collected.The apoptosis rate of urethral striated muscle cells was detected using TUNEL staining.Pathological changes in urethral striated muscle cells were observed by HE staining,Masson staining,and electron microscopy.Results:After bilateral ovariectomy in 17 SD rats,12 tested positive in the urine sneeze test.After intervention,urine leakage symptoms were reduced or disappeared in the electroacupuncture group,urine leakage symptoms were persisted in the model group,and were urine leakage symptoms absent in the blank group.HE and Masson staining results showed that in the blank group,urethral striated muscle cells were plump,neatly arranged,lightly stained,and collagen fibers were compact and orderly.In the model group,muscle cells were significantly atrophied,loosely and disorderly arranged,unevenly and deeply stained,collagen fibers were disordered,and some muscle cells had obvious cracks.These phenomena were alleviated in the electroacupuncture group.TUNEL staining results showed that the apoptosis rates of striated muscle cells in the blank group,model group,and electroacupuncture group were 9.0%,43.5%,and 22.3%,respectively,with statistically significant differences(P<0.01).Conclusion:Electroacupuncture stimulation at Zhongliao(BL33)and Huiyang(BL35)acupoints can alleviate urine leakage symptoms,reduce the apoptosis rate of urethral striated muscle cells,mitigate striated muscle fiber atrophy,and increase the elasticity of collagen fibers in SUI rats.关键词
电针/卵巢去势/压力性尿失禁/尿道横纹肌Key words
Electroacupuncture/Ovariectomy/Stress urinary incontinence/Urethral striated muscle引用本文复制引用
张晓菁,蒋家统,林万庆,鄢霞..电针会阳、中髎穴对压力性尿失禁大鼠尿道横纹肌细胞的影响[J].中医药信息,2025,42(10):17-22,6.基金项目
福建省中青年教师教育厅课题项目(JAT210173) （JAT210173）
