中医药信息2025，Vol.42Issue(10)：23-27,5.DOI:10.19656/j.cnki.1002-2406.20251005
甘草泻心汤对白塞病小鼠肠道菌群及IL-17A干预作用的研究
Study on the Intervention Effect of Gancao Xiexin Decoction on Intestinal Flora and IL-17A in Behcet's Disease Mice
摘要
Abstract
Objective:To study the intervention effect of Gancao Xiexin Decoction on intestinal flora and IL-17A in Behcet's disease mice.Methods:Twenty-four BALB/C mice aged 4-6 weeks were randomly divided into four groups:blank group,model group,traditional Chinese medicine(TCM)group,and positive drug group,with 6 mice in each group.Except for the blank group,mice in the other groups were sensitized with shrimp tropomyosin to establish a Behcet's disease mouse model.After successful modeling,the TCM group was given Gancao Xiexin Decoction at 0.163 8 g/20 g by gavage,the positive drug group was given Bifidobacterium tetravaccine tablets at 0.011 17 g/20 g by gavage,and the blank group and model group were given normal saline by gavage.On the 10th day of gavage administration,fresh feces of the mice were collected for intestinal flora detection using the plate counting method,and the serum IL-17A level was measured by enzyme-linked immunosorbent assay(ELISA).Results:Compared with the blank group,the contents of Bifidobacterium and Lactobacillus in the feces of the model group were significantly decreased(P<0.01),while the contents of Escherichia coli and Enterococcus were significantly increased(P<0.01).Compared with the model group,the contents of Bifidobacterium and Lactobacillus in the feces of the TCM group and positive drug group were significantly increased(P<0.01),while the contents of Escherichia coli and Enterococcus were significantly decreased(P<0.01).ELISA results showed that compared with the control group,the peripheral blood IL-17A level in the model group was significantly increased(P<0.01).Compared with the model group,the serum IL-17A levels in the TCM group and positive drug group were significantly decreased(P<0.01).Conclusion:Gancao Xiexin Decoction can reduce the number of Escherichia coli and Enterococcus in the intestines of Behcet's disease mice,increase the number of Bifidobacterium and Lactobacillus,and inhibit the release of the inflammatory factor IL-17A,thereby exerting a therapeutic effect on Behcet's disease.关键词
甘草泻心汤/白塞病/肠道菌群/白细胞介素-17AKey words
Gancao Xiexin Decoction/Behcet's disease/Intestinal flora/IL-17A引用本文复制引用
姜楠,辛昊洋,车祥晴,王烁,韩海彦..甘草泻心汤对白塞病小鼠肠道菌群及IL-17A干预作用的研究[J].中医药信息,2025,42(10):23-27,5.基金项目
天津中医药大学第二附属医院"青苗计划"项目(QMJH202115) （QMJH202115）
