中医药信息2025，Vol.42Issue(10)：28-35,8.DOI:10.19656/j.cnki.1002-2406.20251006
多指标定量联合PCA、OPLS-DA及CRITIC-TOPSIS评价不同产地银柴胡质量差异
Multi-index Quantitative Evaluation Combined with PCA,OPLS-DA and CRITIC-TOPSIS for Assessing Quality Differences of Stellariae Radix from Different Habitats
摘要
Abstract
Objective:To comprehensively evaluate the quality differences of Stellariae Radix using PCA,OPLS-DA and CRITIC-TOPSIS methods.Methods:The contents of vanillic acid,isoquercitrin,baicalein,wogonin,quercetin,kaempferol,dichotomine H,dichotomine B,dichotamine C,α-spinasterol,β-sitosterol,stigmasterol,stigmast-7-enol,alcohol-soluble extract,total ash and acid-insoluble ash in Stellariae Radix were selected as quality evaluation indicators.PCA and OPLS-DA methods were used to classify 15 batches of Stellariae Radix and select quality differential markers.The CRITIC method was used to calculate the weight of each indicator,and the TOPSIS method was employed to construct a comprehensive quality evaluation model for Stellariae Radix.Results:The contents of 16 indicators in Stellariae Radix from different habitats varied significantly.PCA extracted 2 principal components,representing 85.001%of the information from 16 indicators.OPLS-DA analysis basically divided the 15 batches of Stellariae Radix into 3 groups according to different habitats,and selected quercetin,baicalein,vanillic acid,stigmast-7-enol,dichotomine B and stigmasterol as quality differential markers.The CRITIC-TOPSIS results showed that the maximum relative closeness degree of comprehensive quality evaluation indicators for the 15 batches was 0.6297,and the minimum was 0.3337.Samples from Ningxia and Gansu ranked higher.Conclusion:The quality of Stellariae Radix varies among different habitats.The comprehensive analysis methods of PCA,OPLS-DA and CRITIC-TOPSIS with multiple indicators can be used for quality difference evaluation of Stellariae Radix.关键词
银柴胡/高效液相色谱法/化学计量学/CRITIC/TOPSIS/综合评价Key words
Stellariae Radix/HPLC/Chemometrics/CRITIC/TOPSIS/Comprehensive evaluation引用本文复制引用
杨丽,汪晶,王晓波,孙玉锋..多指标定量联合PCA、OPLS-DA及CRITIC-TOPSIS评价不同产地银柴胡质量差异[J].中医药信息,2025,42(10):28-35,8.基金项目
国家中医药管理局全国中药特色技术传承人才培训项目(国中医药人教函[2023]96号) （国中医药人教函[2023]96号）
