中医药信息2025，Vol.42Issue(10)：36-40,5.DOI:10.19656/j.cnki.1002-2406.20251007
黄花白及对NS398诱导胃黏膜上皮细胞的保护作用研究
Protective Effect of Bletilla ochracea on NS398-Induced Gastric Mucosal Epithelial Cells
摘要
Abstract
Objective:To investigate the protective effect of Bletilla ochracea on NS398-induced human gastric mucosal epithelial cells(GES-1).Methods:Rats were orally administered Bletilla ochracea aqueous extract(2.7 g/kg)for 3 consecutive days,and blood was collected and centrifuged to prepare high-dose drug-containing serum.The CCK-8 method was used to select the optimal modeling time for NS398-induced GES-1 cell damage,the optimal concentration of positive drugs,and the optimal intervention concentration of Bletilla ochracea and medicated serum.Cell viability was measured to determine the optimal therapeutic concentration of the drug-containing serum.Western blot was used to detect the protein expression levels of Bcl-2,Caspase-3,and Bax.Results:The optimal concentration of NS398 for establishing the GES-1 cell injury model after 24 h of treatment was 150 μmol/L.The positive drug U0126 showed the highest cell viability at a concentration of 15 μmol/L,while 1%Bletilla ochracea drug-containing serum significantly improved GES-1 cell viability.Western blot results demonstrated that 1%Bletilla ochracea drug-containing serum significantly reduced the protein expression of Bax and Caspase-3.Conclusion:Bletilla ochracea drug-containing serum exhibits a significant protective effect against apoptosis in NS398-treated GES-1 cells.关键词
黄花白及/胃溃疡/细胞凋亡Key words
Bletilla ochracea/Gastric ulcer/Apoptosis引用本文复制引用
唐秀胜,曾奇,方镕泽..黄花白及对NS398诱导胃黏膜上皮细胞的保护作用研究[J].中医药信息,2025,42(10):36-40,5.基金项目
贵州省科技计划项目(黔科合支撑[2020]4Y075号) （黔科合支撑[2020]4Y075号）
遵义市科技计划项目(遵市科合HZ字[2022]433号) （遵市科合HZ字[2022]433号）
