中医药信息2025，Vol.42Issue(10)：41-45,5.DOI:10.19656/j.cnki.1002-2406.20251008
基于PI3K/Akt信号通路探讨川芎嗪对缺血性脑卒中的抗凋亡作用
Anti-Apoptotic Effect of Ligustrazine on Ischemic Stroke via PI3K/Akt Signaling Pathway
摘要
Abstract
Objective:To investigate the effects of ligustrazine(TMP)on PI3K/Akt signaling pathway and apoptotic protein expression in rat's brain with ischemic stroke(IS).Methods:SD rats were randomly divided into sham group,model group,TMP group,and nimodipine group(n=12 each).Middle cerebral artery occlusion(MCAO)models were established using modified suture-occlusion method.The TMP group received TMP(50 mg/kg),nimodipine group received nimodipine(50 mg/kg),while sham and model groups received equalvolume saline,all groups administered intraperitoneally once daily for 1 week.Neurological deficits were assessed using Longa score.ROS levels in ischemic areas were measured by DCFH-DA fluorescent probe.Western blot detected PI3K/Akt signaling pathway and apoptotic proteins(Bax,Caspase-3)in ischemic brain tissues.Results:Compared with sham group,the model group showed severe neurological impairment(P<0.001).Compared with model group,both TMP and nimodipine groups exhibited:reduced neurological scores(P<0.01),decreased the content of ROS(P<0.01),increased PI3K/Akt protein expression(P<0.01),and downregulated Bax/Caspase-3(P<0.01).Compared with nimodipine group,the TMP group demonstrated:lower neurological scores(P<0.05),reduced ROS(P<0.05),higher PI3K/Akt expression(P<0.05),and decreased the protein expression of Bax/Caspase-3(P<0.05).Conclusion:TMP inhibits apoptotic protein expression post IS,exerting anti apoptotic effects potentially through PI3K/Akt pathway modulation.关键词
川芎嗪/缺血性脑卒中/凋亡/PI3K/Akt信号通路Key words
Ligustrazine/Ischemic stroke/Apoptosis/PI3K/Akt signaling pathway引用本文复制引用
李琦,王岚宁,龙欣悦,符瑶,荆文馨,杨春壮,徐传冰,林强..基于PI3K/Akt信号通路探讨川芎嗪对缺血性脑卒中的抗凋亡作用[J].中医药信息,2025,42(10):41-45,5.基金项目
黑龙江省卫生健康委科技计划项目(20220101020614) （20220101020614）
黑龙江省省属高等学校基本科研业务费科研项目(2023-KYYWF-0938,2022-KYYWF-0665) （2023-KYYWF-0938,2022-KYYWF-0665）
