安徽工程大学学报2025，Vol.40Issue(4)：18-24,7.
TA-TiⅣ涂层包覆大孔吸附树脂固定化猪肝酯酶合成丁酸乙酯
Immobilization of Pig Liver Eesterase on Macroporous Resin Followed by TA-TiⅣ Coating and Application for Synthesis of Ethyl Butyrate
摘要
Abstract
In this research,pig liver esterase was adsorbed using macroporous adsorbent resins and immobilized by TA-TiⅣ nano coatings formed by tannic acid and dihydroxybis(ammonium lactato)titanium(Ⅳ)solution,and the enzymatic properties of immobilized pig liver esterase were investigated.Then the immobilized pig liver esterase was used to catalyze esterification of butyric acid and ethanol for the synthesis of ethyl butyrate in organic solvent.The factors affecting esterification rate were investigated.The results showed that the esterification rate reached 84.51％after 30 h of reaction with n-heptane as the reaction medium,butyric acid and ethanol at the concentration of 0.1 mol/L,molecular sieve at the addition of 0.09 g/mL,enzyme at the addition of 0.2 g/mL,and the reaction temperature of 40℃.And after repeated use for 4 times,the esterification rate was still 54.21％.关键词
猪肝酯酶/丁酸乙酯/固定化/生物催化Key words
pig liver esterase/ethyl butyrate/immobilization/biocatalysis分类
生物工程引用本文复制引用
程瑶纳,张呈彦,郑瑞,高碧云,魏胜华..TA-TiⅣ涂层包覆大孔吸附树脂固定化猪肝酯酶合成丁酸乙酯[J].安徽工程大学学报,2025,40(4):18-24,7.基金项目
安徽省高等学校自然科学研究项目(KJ2021A0512) （KJ2021A0512）
