亚热带农业研究2025，Vol.21Issue(3)：145-153,9.DOI:10.13321/j.cnki.subtrop.agric.res.2025.03.001
阿特拉津降解真菌Candida tropicalis的分离鉴定与降解特性
Isolation,identification,and degradation characterization of an atrazine-degrading fungus Candida tropicalis
摘要
Abstract
[Purpose]To screen atrazine-degrading fungal strains from the hot and humid soil environment in southern China and explore the degradation characteristics,thereby providing candidate strains for the microbial remediation of atrazine-contaminated soil.[Method]Atrazine-degrading strains were isolated from the farmland soil in Xiamen via the enrichment culture method and then identified through gene sequencing.Various factors,including temperature,pH,inoculum volume,initial atrazine concentra-tion,and carbon sources,were selected for single factor experiments to study the degradation characteristics of the strain.[Result]An atrazine-degrading strain AX1 was screened out.It exhibited round white opaque colonies with an entire margin and smooth tex-ture,and the cells had no flagella.Through morphological observation and genetic sequencing,the strain was identified as Candida tropicalis,a fungal species.The optimal temperature,pH,and carbon source for atrazine degradation by AX1 were 30℃,pH 6-8,and glucose,respectively.After being cultured under the above conditions for 72 h,the strain degraded 49.67%of initial atrazine at 20 mg·L-1 and 42.26%at 50 mg·L-1.[Conclusion]A fungal strain AX1,demonstrating atrazine-degrading capacity,is isolated from the hot and humid soil in southern China,expanding the microbial resources for atrazine degradation.关键词
阿特拉津/热带假丝酵母菌/微生物降解/富集培养Key words
atrazine/Candida tropicalis/microbial degradation/enrichment culture分类
环境科学引用本文复制引用
于海玲,李妍,王明元,彭汇涵,陈其煌,林永华..阿特拉津降解真菌Candida tropicalis的分离鉴定与降解特性[J].亚热带农业研究,2025,21(3):145-153,9.基金项目
福建省厦门市科技计划项目"厦门市菜园土壤三嗪类除草剂系统调查及微生物治理研究与示范"(3502Z20226030). （3502Z20226030）
