亚热带农业研究2025，Vol.21Issue(3)：154-162,9.DOI:10.13321/j.cnki.subtrop.agric.res.2025.03.002
SRB的工业扩培及其对水稻Cd和Pb累积的抑制
Optimization of industrial culture conditions of sulfate-reducing bacteria and their application in the inhibition for Cd and Pb accumulation in rice grains
摘要
Abstract
[Purpose]To optimize the industrial culture conditions of sulfate-reducing bacteria(SRB)and evaluate the inhibitory effects of industrially fermented SRB on cadmium(Cd)and lead(Pb)accumulation in rice grown in paddy fields.[Method]The culture conditions including carbon source,nitrogen source,inorganic salt mass concentration,and pH of a SRB strain YWC001 in an industrial-scale fermenter were optimized,and the optimal industrial fermentation parameters were obtained.Furthermore,plot trials were conducted in paddy fields to assess the inhibitory effects of the industrial fermentation broth of YWC001 on Cd and Pb up-take in rice plants.[Result]Strain YWC001 achieved optimal growth under the conditions of 10 g·L-1 molasses,10 g·L-1 corn steep liquor,10 g·L-1 NaCl,and pH 7.0,with the concentration reaching approximately 12.6×109 CFU·mL-1.Application of the industrial fermentation broth of YWC001 to moderately Cd-contaminated paddy soil planted with the rice cultivar Yongyou 15 in-creased the rice yield by 12%,while decreasing the Cd and Pb concentrations in rice grains by 30.9%and 56.9%,respectively.[Conclusion]Strain YWC001 can be cultured cost-effectively on an industrial scale.Strain YWC001 in industrial fermentation sig-nificantly alleviated Cd and Pb toxicity in rice plants,enhanced the grain yield,and inhibited the uptake and accumulation of Cd and Pb,demonstrating considerable application potential.关键词
硫酸盐还原菌/工业发酵/镉铅污染物/水稻/迁移累积Key words
sulfate-reducing bacteria/industrial fermentation/cadmium and lead pollutants/rice/migration and accumulation分类
环境科学引用本文复制引用
陈远锋,吴永晨,卓桂华,刘先荣,班明玉,杨桂娣..SRB的工业扩培及其对水稻Cd和Pb累积的抑制[J].亚热带农业研究,2025,21(3):154-162,9.基金项目
福建省属公益类科研院所基本科研专项项目"硫酸盐还原菌扩培技术优化及其在农田重金属污染修复中的应用研究"(2024R1015002). （2024R1015002）
