分子影像学杂志2025，Vol.48Issue(10)：1191-1197,7.DOI:10.12122/j.issn.1674-4500.2025.10.01
基于半乳糖胺的介孔二氧化锰双模态肝细胞癌纳米探针的制备与实验验证
Preparation and experimental validation of gallamine-based mesoporous manganese oxide dual-modal nanoprobes for hepatocellular carcinoma
摘要
Abstract
Objective This research focused on the synthesis and characterization of a dual-modal nanoprobe(Gal-MnO2/CDDP@PDA-Cy5.5)for fluorescence and magnetic resonance imaging of hepatocellular carcinoma.The study further explored the probe's specific targeting efficacy against ASGPR-expressing Huh-7 cells and evaluated its performance in magnetic resonance imaging through in vitro experiments.Methods Potassium permanganate solution was added to silica and etched with anhydrous sodium carbonate.The resulting solution was conjugated with polydopamine(DA),galactosamine(Gal),and fluorescent Cy5.5 to obtain Gal-MnO2@PDA-Cy5.5.Cisplatin(CDDP)was introduced into the solution,followed by overnight incubation at room temperature and subsequent centrifugation to remove unreacted CDDP.Cytotoxicity and cellular uptake were assessed using the CCK-8 assay and flow cytometry,respectively.The relaxation rate was measured by Niumag small-scale NMR spectroscopy,and the enhancement degree was evaluated using magnetic resonance imaging.Results The prepared nanoprobes,as observed by transmission electron microscopy,exhibited uniform size and granular morphology with a particle size of 185.0±6.3 nm.The hydrodynamic diameter was measured to be 185.1±16.4 nm,with a zeta potential of 22.5±0.3 mV and a relaxivity of 14.589(mmol/L)-1s-1.The magnetic resonance imaging signal intensity progressively enhanced with increasing nanoprobe concentration.Cytotoxicity assays demonstrated minimal toxicity of the nanoprobes.Conclusion This study successfully synthesized a dual-modal nanoprobe targeting ASGPR,designated as Gal-MnO2/CDDP@PDA-Cy5.5,which demonstrates specific binding to target cells in vitro.Validation results confirmed that the synthesized nanoprobe exhibits excellent stability and biosafety,along with specific targeting capability toward Huh-7 cells.Furthermore,it possesses imaging functionality that enhances T1 contrast in magnetic resonance imaging.关键词
半乳糖胺/介孔二氧化锰/双模态/纳米探针/制备/实验研究Key words
galactosamine/mesoporous manganese dioxide/dual-modal/nanoprobes/preparation/experimental study引用本文复制引用
胡海峰,曹颖,王影,王梦娇,王余广,郝利国,肖辉宇..基于半乳糖胺的介孔二氧化锰双模态肝细胞癌纳米探针的制备与实验验证[J].分子影像学杂志,2025,48(10):1191-1197,7.基金项目
黑龙江省卫生健康委科研课题(20240909010090) （20240909010090）
齐齐哈尔医学院研究生创新基金(QYYCX2024-71) （QYYCX2024-71）
