神经损伤与功能重建2025，Vol.20Issue(11)：621-626,633,7.DOI:10.16780/j.cnki.sjssgncj.20240265
自然衰老小鼠的性别依赖性行为改变
Gender-dependent Behavioral Alterations in Natural Aged Mice
摘要
Abstract
Objective:To investigate the changes in motor function,sensory function,cognitive function,and fine behavior in male and female mice during natural aging.Methods:C57BL/6J mice aged 3 months and 21 months were subjected to assessments of motor function(open-field test,rotarod test,balance beam test,and gait analysis),sensory function(Von Frey test),cognitive function(novel object recognition test),and fine behavior analysis in home cages to compare the effects of natural aging on the behavior of male and female mice.Results:In motor function tests,compared with younger mice of the same sex,elderly female mice exhibited a significant decrease in both the distance traveled and movement speed in the open field(P<0.05),while no significant changes were observed in elderly male mice.In the rotarod test,both elderly male and female mice showed a significant decline in motor balance(P<0.01).In the balance beam test,the motor balance and coordination of elderly mice deteriorated,with a more pronounced decline in elderly male mice(P<0.0001).In the gait analysis,both elderly male and female mice demonstrated a reduction in gait symmetry(P<0.001).Regarding sensory function,the pain thresholds of both elderly male and female mice were significantly higher than those of younger mice of the same sex(P<0.0001),indicating a diminished response to noxious stimuli.In terms of cognitive function,elderly male and female mice lost the preference for exploring novel objects that was observed in younger mice.The results of fine behavior tests in home cages revealed that,compared with younger mice of the same sex,elderly female mice showed a 9.14%decrease in spontaneous motor behavior and a 38.05%decrease in exploratory behavior,while self-care behavior increased by 15.52%.In elderly male mice,spontaneous motor behavior and exploratory behavior decreased by 46.50%and 9.59%,respectively,while self-care behavior increased by 2.36%.Compared with male mice,female mice spent a greater proportion of time on exploratory and spontaneous motor behaviors and engaged in less self-care behavior overall.Conclusion:As mice age naturally,their motor,sensory,and cognitive functions decline,exhibiting certain sex-dependent characteristics.关键词
小鼠/衰老/性别/行为/家笼内精细行为Key words
mice/aging/gender/ethology/home-cage behaviors分类
临床医学引用本文复制引用
王秋颖,荆瀛黎,孙明明,李婷,颜义彤,于艳..自然衰老小鼠的性别依赖性行为改变[J].神经损伤与功能重建,2025,20(11):621-626,633,7.基金项目
中央级公益性科研院所基本科研业务费专项资金项目(基于肠道微生物研究老龄化小鼠脊髓损伤的病理生理改变和机制,No.2022CZ-5 （）
短链脂肪酸对脊髓损伤小鼠神经功能修复的实验研究,No.2021CZ-8) （）
