工业微生物2025，Vol.55Issue(5)：179-184,6.DOI:10.3969/j.issn.1001-6678.2025.05.044
有氧呼吸对乳球菌发酵合成乳酸链球菌素的影响
Effects of Aerobic Respiration on the Nisin Production by Lactococcus Lactis
摘要
Abstract
To improve the efficiency of lactic acid bacteria in the aerobic fermentation and synthesis of nisin,the effects of aerobic respiration cofactors(heme,VB1,and VB7)on cell growth and nisin synthesis were investigated.Through orthogonal experiments,the optimal concentrations ofthe three cofactors were determined tobe 3 μg/mL,100 μmol/mL,and 10 μmol/mL.After 20 hours of anaerobic culture,the biomass of Lactococcus lactis HB03 reached its peak(OD600=12.0),but the cells started to autolyze,reducing by 28%to an OD600 of 8.66 at 45 hours.In contrast,with staged aerobic culture(24 hours of aerobic cultivation and adding optimized concentrations of cofactors),the biomass of the lactococcus continued to grow,OD600 reaching 14.23 at 34 hours,an 18.5%increase in peak biomass compared to the control(anaerobic cultivation).Moreover,compared to the rapid autolysis seen in the control after the 20 hour peak biomass,the biomass in staged aerobic culture remained stable,and OD600 is approximately 14.2 from 34 to 45 hours,showing significantly enhanced cell activity stability.Meanwhile,the nisin titer in the anaerobic culture peaked at 6 458 IU/mL at 18 hours and continuously degraded to 3 571 IU/mL at 34 hours.In the staged aerobic culture,the nisin titer synthesized continuously from 24 to 34 hours,reaching a peak of 7 395 IU/mL,which was a 14.5%increase over the control's peak and a 102%increase in titer during the same period.Aerobic respiration significantly improved the biomass and nisin synthesis efficiency of Lactococcus lactis,providing a new approach for industrial production.关键词
乳酸乳球菌/乳酸链球菌素/有氧呼吸Key words
Lactococcus lactis/nisin/aerobic respiration引用本文复制引用
龚宇杰,魏丽雅,李沛,李库,王志,陈雄..有氧呼吸对乳球菌发酵合成乳酸链球菌素的影响[J].工业微生物,2025,55(5):179-184,6.基金项目
宜昌市科技研究与开发项目(A23-4-001). （A23-4-001）
