菌物学报2025，Vol.44Issue(11)：194-209,16.DOI:10.13346/j.mycosystema.250109
撕裂耙齿菌生物学特性及利用竹屑驯化栽培
Biological characteristics of Irpex laceratus and domesticated cultivation using bamboo shavings
摘要
Abstract
A strain of Irpex was isolated from Auricularia cornea-cultivated substrate using banboo shavings.Through morphological observation and ITS sequencing analysis,the strain was identified as Irpex laceratus.The orthogonal experiment results indicated that the main factor affecting the mycelial growth of I.laceratus was the nitrogen source,followed by the carbon source,pH and temperature.The optimal carbon to nitrogen ratio is 50:1-52:1.Addition of 14.5%-43.5%bamboo shavings significantly accelerated mycelial growth.The content of active substances varied with different formulations of cultivated substrates.Formula B(wood shavings 29%,bamboo shavings 29%,wheat bran 20%,cottonseed hulls 20%,lime 1%,gypsum 1%)exhibited the highest total triterpene content(66.10 mg/g);formula I(bamboo shavings 78%,wheat bran 20%,lime 1%,gypsum 1%)showed the highest total protein content(21.83%);formula CK(wood chips 58%,wheat bran 20%,cottonseed husks 20%,lime 1%,gypsum 1%)exhibited the highest total flavonoid content(0.99 mg/g);formula C(wood chips 14.5%,bamboo chips 43.5%,wheat bran 20%,cottonseed husks 20%,lime 1%,gypsum 1%)had the highest polysaccharide content of 2.80 g/100 g;formula F(bamboo chips 68%,wheat bran 20%,cottonseed husks 10%,lime 1%,gypsum 1%)demonstrated the highest total phenol content of 19.02 mg/g.The results provide valuable reference data for the cultivation and medicinal development of I.laceratus.关键词
药用真菌/正交试验/毛竹竹屑/人工栽培/活性物质Key words
medicinal fungi/orthogonal experiment/Moso bamboo chips/artificial cultivation/active substance引用本文复制引用
李东晨,王欣,张望舒,茆卫琳,张磊,张健,王保玺,刘晓草,陈胜,谢锦忠..撕裂耙齿菌生物学特性及利用竹屑驯化栽培[J].菌物学报,2025,44(11):194-209,16.基金项目
2022安吉县绿色竹产业创新服务综合体重点研发项目 （）
宁波市科技计划项目(2019B10020) （2019B10020）
科技对口支援和东西部协作项目(2022C04013) （2022C04013）
浙江省省院合作林业科技项目(2020SY15)This work was supported by the2022Anji County Green Bamboo Industry Innovation Service Complex Key Research and Development Project,the Ningbo Science and Technology Program Project(2019B10020),the Science and Technology Counterpart Support and East-West Collaboration Project(2022C04013),and the Zhejiang Province-Institute Cooperation Forestry Science and Technology Project(2020SY15). （2020SY15）
