菌物学报2025，Vol.44Issue(11)：223-235,13.DOI:10.13346/j.mycosystema.250097
不同木质纤维素基质对白腐真菌固态发酵产漆酶的影响
Effects of different lignocellulosic substrates on laccase production by white-rot fungi in solid-state fermentation
摘要
Abstract
Twenty-four newly isolated strains of white-rot fungi were cultured on guaiacol-supplemented medium for screening laccase-producible strains,the capacity of laccase production of the 12 strains obtained was analysed in submerged fermentation and solid-state fermentation on different lignocellulosic substrates.The results indicated that there was no significant correlation between the laccase activity detected under submerged fermentation conditions and the ratio of colony diameters to photochromic laps among the 12 test strains(P>0.05).Similarly,no significant association was observed between the laccase activity detected during solid-state fermentation on different lignocellulosic substrates and the ratio of colony diameters to photochromic laps for the various test strains(P>0.05).In submerged fermentation,Cerrena unicolor LKY 798,Trametes hirsuta LKY 831,Funalia trogii LKY 846,and Neofavolus alveolaris LKY 850 exhibited comparatively high laccase activities keeping for a fairly long duration.N.alveolaris LKY 850 and Trametes sp.LKY 797 demonstrated the strongest laccase production capacities under solid-state fermentation condition using leaves of Quercus variabilis,Populus beijingensis,and needles of Pinus tabuliformis as substrates.It seems that leaves of Quercus variabilis and Populus beijingensis are more suitable than needles of Pinus tabuliformis for laccase production under solid-state fermentation condition.Additionally,Trametes sp.LKY 797,F.trogii LKY 846,and N.alveolaris LKY 850 showed relatively good laccase activities in fermentation using needles of Pinus tabuliformis as the substrate,with maximum laccase activities exceeding 3 700 U/mL.关键词
愈创木酚/液态发酵/阔叶树/油松松针/漆酶活性Key words
guaiacol/submerged fermentation/broad-leaved tree/needles of Pinus tabuliformis/laccase activity引用本文复制引用
边禄森,安琪,刘慧慧,苗朔,杨依宁,李施漫,王兴红,韩美玲..不同木质纤维素基质对白腐真菌固态发酵产漆酶的影响[J].菌物学报,2025,44(11):223-235,13.基金项目
中国林科院中央级公益性科研院所基本科研业务费(CAFYBB2021MA007) （CAFYBB2021MA007）
河北省自然科学基金(C2024408007)This work was supported by the Fundamental Research Funds for the Central Non-profit Research Institution of the Chinese Academy of Forestry(CAFYBB2021MA007)and the Hebei Province Natural Science Foundation(C2024408007). （C2024408007）
