菌物学报2025，Vol.44Issue(11)：236-249,14.DOI:10.13346/j.mycosystema.250129
云芝-太子参双向发酵全性基质的配方优化和生物活性分析
Optimization of a medicinal and nutritional substrate for bi-directional solid-state fermentation of Trametes versicolor-Pseudostellaria heterophylla and its bioactivity analysis
摘要
Abstract
To improve the utilization of Pseudostellaria heterophylla by-product resources and broaden the sources of feed,the bi-directional solid-state fermentation of medicinal P.heterophylla and Trametes versicolor was carried out.The substrate formula was optimized using mixture design with the mycelial growth rate as response value.The change in the content of bioactive compositions was assayed during the mycelial post-maturation stage.The content of nutritional and bioactive compositions and antioxidant activities of the fermented substrate were determined.The optimal substrate consisted of 59%corncob,10%P.heterophylla roots,29%wheat bran,1%gypsum,and 1%lime.The content of polyphenols,flavonoids,polysaccharopeptide,and polysaccharides in the substrate increased with time during the mycelial post-maturation stage,while the triterpenoids decreased first and then increased.In scaled-up fermentation in plastic bags,the crude protein content of the fermented substrate was 8.20%,or increased by 31.41%as compared with that of the non-fermented substrate(6.24%).The crude ash content rose from 3.50%to 8.10%or increased by 131.43%.The content of polyphenols reached 6.87 mg/g,8.70%higher than that of the non-fermented control(6.32 mg/g).The neutral detergent fiber content decreased considerably,while the acidic detergent fiber increased.The scavenging rate of ethanol extract of the fermented substrate against DPPH radicals was almost close to that of the non-fermented control,while that of the water extract was lower than that of the control.ABTS radical scavenging rate of both the water and ethanol extracts of the fermented substrate was lower than that of the non-fermented substrate.The present study provides a useful reference for the utilization of P.heterophylla by-product resources and the development of the fermented substrate as animal feed.关键词
药用真菌/云芝/太子参/混料设计/中草药发酵/饲料Key words
medicinal mushroom/Trametes versicolor/Pseudostellaria heterophylla/mixture design/Chinese herbal medicine fermentation/feed引用本文复制引用
杨义辉,阿依古力·阿不都克力木,叶祖云,郑世仲,魏奇,黄千慧,张维瑞,刘盛荣..云芝-太子参双向发酵全性基质的配方优化和生物活性分析[J].菌物学报,2025,44(11):236-249,14.基金项目
福建省科技厅对外合作项目(2021I0046)This work was supported by the Foreign Cooperation Program of Science and Technology Department of Fujian Province(2021I0046). （2021I0046）
