菌物学报2025，Vol.44Issue(11)：250-265,16.DOI:10.13346/j.mycosystema.250113
镉胁迫环境接种两种暗色有隔内生真菌对甘草生长和药用成分含量的影响
Effects of inoculation with two species of dark septate endophytes on the growth and content of medicinal components of Glycyrrhiza uralensis under cadmium stress
摘要
Abstract
Dark septate endophytes(DSE)widely colonize the roots of plants in heavy metal-contaminated soils.The effects of DSE on the growth and medicinal components of Glycyrrhiza uralensis at different growth stages require further elucidation.In this study,pot soil containing different concentrations of cadmium(Cd)(0,5,10,and 15 mg/kg)was inoculated with Neocamarosporium betae(NB)and Cladosporium fusiforme(CF)to explore the effects of DSE inoculation on the growth and medicinal components of G.uralensis.The results showed that both CF and NB could effectively colonize the roots of G.uralensis.DSE inoculation increased the total root length,root volume,root surface area,and biomass of G.uralensis,as well as the content of available nitrogen in the soil.DSE colonization resulted in increase of soluble protein and glutathione levels in G.uralensis and decrease of malondialdehyde content.Variance partitioning analysis indicated that DSE explained 46.60%,13.60%,and 7.80%of the variation of soil factors in the early,middle,and late stages respectively.Similarly,DSE accounted for 52.20%and 40.00%of the physiological variations of G.uralensis in the early and late stages,respectively.Soil factors contributed to 22.00%,38.30%,and 58.40%of the variation of G.uralensis medicinal components in the three growth stages.Available potassium,phosphorus,and nitrogen in the soil significantly enhanced the biomass and root length of G.uralensis in the early stage.These data suggest that cadmium stress inhibited the growth of G.uralensis,while DSE inoculation could improve soil nutrient status and promote G.uralensis growth,and thereby facilitate the accumulation of glycyrrhizin and glycyrrhizic acid.关键词
暗色有隔内生真菌/镉污染土壤/生长性状/药用成分含量/甘草Key words
dark septate endophytes/Cd-contaminated soil/growth status/medicinal ingredient/Glycyrrhiza uralensis引用本文复制引用
付欣欣,张晓静,豆浩然,陈文静,李春苗,贺学礼..镉胁迫环境接种两种暗色有隔内生真菌对甘草生长和药用成分含量的影响[J].菌物学报,2025,44(11):250-265,16.基金项目
河北省自然科学基金(H2022201056) （H2022201056）
河北大学研究生创新能力培养资助项目(HBU2024BS004)This work was supported by the Hebei Natural Science Foundation(H2022201056)and the Project for Cultivating Postgraduate Innovation Ability of Hebei University(HBU2024BS004). （HBU2024BS004）
