菌物学报2025，Vol.44Issue(11)：266-275,10.DOI:10.13346/j.mycosystema.250101
镰孢菌ZH-H2修复HMW-PAHs污染老化土壤的关键参数优化
Optimization of key parameters for remediation of aging soil contaminated by HMW-PAHs using Fusarium sp.ZH-H2
摘要
Abstract
Remediation of aged soil contaminated by high molecular weight polycyclic aromatic hydrocarbons(HMW-PAHs)in a typical coal mining area was studied,using Fusarium sp.ZH-H2 as the remedial strain.A three-factor four-level orthogonal experiment was conducted to investigate the remediation efficiency of ZH-H2 under different environmental conditions.The study showed that the optimum environmental parameters for degradation of soil HMW-PAHs by ZH-H2 were 1 000-2 000 mg/kg of inoculation amount,30-40℃of temperature and 5%-15%of humidity.Under these environmental conditions,the total removal range of 10 HMW-PAHs was 22.72%-25.44%.Besides,building upon these optimized conditions,subsequent multi-stage inoculations revealed that both total HMW-PAHs and individual PAH removal rates peaked at the fourth inoculation cycle,achieving a total removal rate of 49.64%and individual removal rates ranging from 45.24%to 59.73%.Notably,benzo(a)pyrene(BaP)exhibited the highest degradation efficiency reaching 59.73%.This study innovatively proposes a ZH-H2-based multi-stage enhanced remediation strategy,establishing an"environmental optimization and inoculation enhancement"multi-stage remediation model for HMW-PAHs contaminated soils.关键词
镰孢菌ZH-H2/接菌次数/HMW-PAHs/环境因子/去除率Key words
Fusarium sp.ZH-H2/inoculation times/high molecular weight polycyclic aromatic hydrocarbons/environmental factor/removal rate引用本文复制引用
祁明月,李家辉,张丽秀,郝瑞,韩芝兰,杨志新..镰孢菌ZH-H2修复HMW-PAHs污染老化土壤的关键参数优化[J].菌物学报,2025,44(11):266-275,10.基金项目
河北省自然科学基金(D2023204018) （D2023204018）
鄂尔多斯市乌兰煤炭(集团)有限责任公司科研课题(ELD-UMC-2023-006) （集团）
华北作物改良与调控国家重点实验室课题(NCCIR2022ZZ-9) （NCCIR2022ZZ-9）
现代农业产业技术体系河北省创新团队建设项目(HBCT2024140209,HBCT2024050203)This work was supported by the Natural Science Foundation of Hebei Province(D2023204018),the Scientific Research Project of Ordos Wulan Coal(Group)Company Limited(ELD-UMC-2023-006),the Project of the State Key Laboratory of Crop Improvement and Regulation in North China(NCCIR2022ZZ-9),and the Hebei Innovation Team Construction Project of Modern Agricultural Industrial Technology System(HBCT2024140209,HBCT2024050203). （HBCT2024140209,HBCT2024050203）
