南京农业大学学报2025，Vol.48Issue(6)：1326-1334,9.DOI:10.7685/jnau.202407027
大豆玉米带状复合种植模式对根际微生物群落结构和多样性的影响
Effects of maize-soybean strip intercropping on rhizosphere microbial community structure and diversity
摘要
Abstract
[Objectives]The purpose of this study was to explore the effects of the maize-soybean strip intercropping system on the rhizosphere microorganisms of soybean and maize,providing a theoretical basis for in-depth understanding of the rhizosphere microbial characteristics in this system,and offering scientific support for developing high-quality green agriculture and optimizing crop system structures.[Methods]The four treatments included SS(soybean monocropping),S-SM(soybean strip intercropping),MM(maize monocropping),and M-SM(maize strip intercropping).Soil samples were collected during the maize tasseling and soybean flowering pod stages,and high-throughput sequencing of Illumina Novaseq 6000 was employed to assess community structure,richness,diversity index,as well as the changes in the dominant soil bacterial and fungal microflora and their relationship with soil physicochemical properties by redundancy analysis.[Results]This study demonstrated that maize-soybean strip intercropping altered the soil microbial community structure and increased the diversity of rhizosphere soil microorganisms.The ACE index,Chao index,Shannon index,and Simpson diversity indices of bacterial diversity in the rhizosphere soil under S-SM,M-SM were significantly higher than those under SS and MM,the Shannon and Simpson fungal diversity indices for the rhizosphere soil with the M-SM system were significantly higher than those under MM,while the S-SM did not significantly differ from those in the SS.The proportions of beneficial microorganisms under the four treatments were 29.18%(SS),29.52%(S-SM),17.09%(MM)and 31.02%(M-SM),respectively,and the ratio of beneficial microorganisms in the rhizosphere soil was altered by maize-soybean strip intercropping,the abundances of Lysobacter,Pseudomonas,and Bacillus were significantly higher in the S-SM system than in SS.Based on the RDA,soil physical and chemical properties were closely related to the composition and diversity of rhizosphere microbial communities.Available nutrients were the main factors affecting the change of phylum,especially pH value and N in soil.[Conclusions]After soybean-maize strip intercropping,the soil microbial diversity index and proportion of beneficial microorganisms increased,and the bacterial and fungal community proportions were altered.Furthermore,bacteria and fungi in the rhizosphere soil under maize-soybean strip intercropping were closely associated with the physicochemical properties of the environment.关键词
大豆/玉米/细菌/真菌/群落结构/多样性/带状间作/根际微生物Key words
soybean/maize/bacteria/fungi/community structure/diversity/strip intercropping/rhizospheric microorganism分类
农业科技引用本文复制引用
陈亚丽,巨亚雯,李美霞,周长勇,付佑胜,曹凯歌,钱新..大豆玉米带状复合种植模式对根际微生物群落结构和多样性的影响[J].南京农业大学学报,2025,48(6):1326-1334,9.基金项目
江苏农业自主创新资金项目[CX(23)3008] （23）
淮安市农科院科研基金项目(HNY202205,HYS202019) （HNY202205,HYS202019）
淮安市自然科学研究计划项目(HAB202374) （HAB202374）
