上海针灸杂志2025，Vol.44Issue(11)：1360-1369,10.DOI:10.13460/j.issn.1005-0957.2025.11.1360
麦粒灸对慢性不可预知温和应激小鼠抑郁样行为的干预效应及机制研究
The intervention effect and mechanism of wheat-grain-sized moxibustion on depressive-like behaviors in mice with chronic unpredictable mild stress
摘要
Abstract
Objective To investigate the mechanism of wheat-grain-sized moxibustion in improving depressive-like behaviors by observing its intervention effect on the behaviors of mice with chronic unpredictable mild stress(CUMS)and the impact on inflammatory responses in the prefrontal cortex.Method Thirty-six C57BL/6J mice were allocated to a control group,a model group,a wheat-grain-sized moxibustion group,and a Fluoxetine group,each consisting of 9 mice.Given the successful establishment of CUMS mouse models,mice in the wheat-grain-sized moxibustion group received wheat-grain-sized moxibustion on a daily basis,and mice in the Fluoxetine group received intraperi-toneal injection of Fluoxetine.The sucrose preference test,tail suspension test,forced swim test,open field test,and elevated plus maze test were adopted for behavioral assessment.Hematoxylin-eosin(HE)staining was used to observe the pathological alterations in the prefrontal cortex.Immunohistochemistry was used to determine the expression of microglial activation marker ionized calcium-binding adaptor molecule 1(Iba-1)in the prefrontal cortex.Enzyme-linked immunosorbent assay and reverse transcription-quantitative polymerase chain reaction(RT-qPCR)were used to measure the mRNA and protein expression levels of interleukin-6(IL-6),interleukin-1β(IL-1β),and tumor necrosis factor-α(TNF-α)in the prefrontal cortex.Result Compared to the control group,mice in the model group exhibited a reduction in the sucrose preference rate(P＜0.05),along with prolonged immobility time in the tail suspension test(P＜0.05)as well as in the swimming test(P＜0.05),a decreased number of entries into the central area and time spent in the central area in the open field test(P＜0.05),and a reduced number of entries into and time spent in the open arms in the elevated plus maze test(P＜0.05).Compared to the model group,mice in the wheat-grain-sized moxibustion and Fluoxetine groups showed an increased sucrose preference rate(P＜0.05)and reduced immobility time in the tail suspension test(P＜0.05)as well as in the swimming test(P＜0.05).Compared to the control group,the number of neurons in the prefrontal cortex of the model group mice declined significantly(P＜0.05),the expression of Iba-1 increased significantly(P＜0.05),the protein expression levels of IL-6,IL-1β,and TNF-α increased markedly(P＜0.05),and the expression of IL-1β mRNA increased(P＜0.05).Compared to the model group,the number of neurons in the prefrontal cortex of mice in the wheat-grain-sized moxibustion and Fluoxetine groups increased significantly(P＜0.05),the expression of Iba-1 decreased notably(P＜0.05),the protein and mRNA expression levels of IL-1β dropped significantly(P＜0.05),and the TNF-α mRNA expression level decreased notably(P＜0.05).Conclusion Wheat-grain-sized moxibustion ameliorates depressive-like behaviors in CUMS mice,potentially through mechanisms involving the suppression of microglial activation in the prefrontal cortex and the alleviation of inflammatory damage.关键词
麦粒灸/慢性不可预知温和应激/抑郁症/炎症/前额叶皮层/小鼠Key words
Wheat-grain-sized moxibustion/Chronic unpredictable mild stress/Depression/Inflammation/Prefrontal cortex/Mice分类
中医学引用本文复制引用
李哲英,杨悠然,阴嘉琪,徐开阳,刘世敏,龚陈媛,胡丹,朱诗国,赵琛..麦粒灸对慢性不可预知温和应激小鼠抑郁样行为的干预效应及机制研究[J].上海针灸杂志,2025,44(11):1360-1369,10.基金项目
国家自然科学基金面上项目(82373908) （82373908）
上海中医药大学科技发展项目自然科学类(23KFL030) （23KFL030）
上海中医药大学针灸推拿学院"腾飞人才培育计划"(2023ZTTF01) （2023ZTTF01）
上海中医药大学大学生创新创业训练计划项目[SHUTCM2023101上中医教务(2023)10号-] （2023）
