山西大学学报（自然科学版）2025，Vol.48Issue(6)：1226-1237,12.DOI:10.13451/j.sxu.ns.2025085
三种杀虫剂对两种鳞翅目害虫生长发育及解毒代谢的影响
The Effects of Three Insecticides on the Growth and Detoxification Metabolism of Two Lepidoptera Pests
摘要
Abstract
Chemical pesticides are the main controlling means of Lepidoptera pests.Research on pest resistance due to the excessive use of chemical pesticides has attracted much attention.In order to evaluate and reveal the insecticidal efficiency and physiological biochemical effects of three chemical insecticides(ebufenozide,cyantraniliprole and indoxacarb)on Spodoptera exigua(S.exigua)and Spodoptera frugiperda(S.frugiperda),the sublethal concentrations of three insecticides were determined by indoor bioassay,and the effects of sublethal concentrations insecticides on key biological parameters were determined,including the survival rate,pu-pation rate,eclosion rate,average body length,average body weight,developmental duration of these two Lepidoptera pests.Addi-tionally,the activity of detoxification enzymes in the larvae were measured using enzyme activity detection kits.It was found that three pesticides could inhibit the growth and development of larvae in varying degrees.Tebufenozide most effectively inhibited lar-val growth in S.exigua,prolonged its developmental period and reduced larval survival by 27.77%compared to the control group.Furthermore,the carboxylesterase activity in S.exigua larvae were significantly higher than that in control group at 12 h,36 h and 48 h post-treatment,while glutathione S-transferase activity in S.exigua larvae were significantly higher than that in control group after 12 h.In contrast,Indoxacarb significantly suppressed larval growth of S.frugiperda,extended its developmental period,and de-creased the larval survival rate by 27.22%compared with the control group.Carboxylesterase activity in S.frugiperda larvae was significantly higher than that in the control group at 12 h,then gradually decreased,and rose again significantly by 48 h,and gluta-thione S-transferase activity in S.frugiperda were significantly higher than that in control group at 24 h and 48 h post-treatment.The results indicated that tebufenozide and indoxacarb exhibit high insecticidal efficiency against S.exigua and S.frugiperda respective-ly,while both can upregulate glutathione S-transferase and carboxylesterase activity in the larvae at different time points.However,the insecticidal efficiency of cyantraniliprole against the two kinds of insects was not as good as that of tebufenozide and indoxacarb.Results from this study can provide scientific basis and reference for the comprehensive control strategy of Lepidoptera pests and the rational application of insecticides.关键词
甜菜夜蛾/草地贪夜蛾/溴氰虫酰胺/茚虫威/虫酰肼Key words
Spodoptera exigua/Spodoptera frugiperda/cyantraniliprole/indoxacarb/tebufenozide分类
农业科技引用本文复制引用
卫丽丽,徐美琳,张瑞洁,付月君..三种杀虫剂对两种鳞翅目害虫生长发育及解毒代谢的影响[J].山西大学学报（自然科学版）,2025,48(6):1226-1237,12.基金项目
山西省基础研究计划资助项目(20240302212066) （20240302212066）
山西省高等学校科技创新项目(2022L473) （2022L473）
山西省科技创新人才团队项目(202204051001035) （202204051001035）
山西省技术创新中心基地项目(202404010920021) （202404010920021）
