新发传染病电子杂志2025，Vol.10Issue(5)：36-40,5.DOI:10.19871/j.cnki.xfcrbzz.2025.05.007
2023年深圳地区182例手足口病和疱疹性咽峡炎患儿肠道病毒优势血清型VP1基因特征分析
VP1 gene characteristics of predominant enterovirus serotype of 182 hand,foot,and mouth disease and herpangina patients from Shenzhen in 2023
摘要
Abstract
Objective To investigate enterovirus serotypes in children with hand,foot,and mouth disease(HFMD)or herpangina(HA)in Shenzhen during 2023,and analyze evolutionary traits of the predominant serotype.Method Fecal sample or throat swabs,collected from 182 HFMD/HA patients in Shenzhen during 2023.Enteroviruses were detected by quantitative real-time PCR,and positive samples were typed via PCR-sequencing;besides,the full-length VP1 sequences of the predominant serotype of enterovirus were obtained for phylogenetic tree construction using MEGA X,and sequence similarity analysis using Bioedit.Result Enterovirus prevalence was 62.1%(113/182),with coxsackievirus A6(CV-A6)as the predominant serotype.Phylogenetic analysis resolved CV-A6 into 4 lineages(A-D),with all 2023 Shenzhen isolates assigned to the D3a subclade of linages D.Nucleotides and amino acid sequence identities with representative strains of lineages A-C were 82.0%-85.1%and 93.7%-96.7%,respectively.Conclusion CV-A6 was the predominant enterovirus serotype in 2023 HFMD/HA pediatric cases from Shenzhen,belonging to the globally circulating D3a subclade of lineage D.关键词
手足口病/疱疹性咽峡炎/柯萨奇病毒A组6型/VP1基因/系统发育进化树Key words
Hand,foot,and mouth disease/Herpangina/Coxsackievirus A6/Viral protein 1/Phylogenetic trees分类
临床医学引用本文复制引用
李盛勇,麦光兴,严泽浩,肖克林..2023年深圳地区182例手足口病和疱疹性咽峡炎患儿肠道病毒优势血清型VP1基因特征分析[J].新发传染病电子杂志,2025,10(5):36-40,5.基金项目
1.深圳市宝安区医学会医疗卫生科研项目(BAYXH2024029) （BAYXH2024029）
2.深圳市宝安区2024年度区属公立医院高质量发展研究项目(BAGZL2024153) （BAGZL2024153）
