新发传染病电子杂志2025，Vol.10Issue(5)：41-46,6.DOI:10.19871/j.cnki.xfcrbzz.2025.05.008
人源诺如病毒感染引起的急性胃肠炎儿童肠道微生物群组成差异分析
Differences in gut microbiota composition in children with acute gastroenteritis due to human norovirus
摘要
Abstract
Objective To investigate the characteristics of gut microbiota in children with symptomatic human norovirus(HNoV)infection and to perform functional analysis.Method Twenty-five preschool children who received treatment for acute gastroenteritis caused by HNoV infection in the Pediatrics Department of Dazu Hospital affiliated to Chongqing Medical University from July 2024 to November 2024 were included as the HNoV group.In addition,25 healthy children who tested negative for HNoV GII gene amplification were included as the control group according to the propensity score matching principle.Fecal samples from both groups were collected to analyze gut microbiota characteristics.Evaluate alpha-diversity,beta-diversity,taxonomic groups with different abundance,and microbial functions through bioinformatics analysis.Result Compared with the control group,the gut microbiota of the HNoV group was enriched in Firmicutes(62.25%vs 57.24%),while the proportions of Actinobacteria(1.57%vs 3.02%)and Bacteroidetes(0.22%vs 1.14%)were relatively reduced,and leading to an increased Firmicutes/Bacteroidetes ratio(11.20%vs 17.21%,P=0.003).Similarly,significant microbial differences were observed at both the family and genus levels between the two groups.The abundances of Vibrio(P=0.006),Pseudomonas(P=0.017),Enterococcus(P=0.020),and Clostridium(P=0.036)in the HNoV group were higher than those in the control group.The differences in these microorganisms were accompanied by genetic variations involved in the biosynthesis,transport,and catabolism of secondary metabolites,particularly an increased function of nitrate reductase and an elevated abundance of alkyl hydroperoxide reductase subunit C,which may contribute to greater susceptibility to HNoV.Conclusion HNoV infection leads to significant reduction in gut microbiota abundance and diversity and significant dysregulation of gut microbiota composition and function in children.关键词
人源人类诺如病毒/肠道微生物群/16S rRNA扩增子焦磷酸测序/功能预测Key words
Human norovirus/Gut microbiota/16S rRNA amplicon pyrophosphate sequencing/Function prediction分类
临床医学引用本文复制引用
王廷婷,雷芳,杨阳,陈岑,周延..人源诺如病毒感染引起的急性胃肠炎儿童肠道微生物群组成差异分析[J].新发传染病电子杂志,2025,10(5):41-46,6.基金项目
重庆市科技发展项目(DZKJ2022JSYJ-KWXM1031) （DZKJ2022JSYJ-KWXM1031）
