针刺研究2025，Vol.50Issue(11)：1248-1256,9.DOI:10.13702/j.1000-0607.20250615
电针对原发性痛经大鼠大麻素相关受体CB2R、TRPV1及脊髓小胶质细胞极化的影响
Effect of electroacupuncture on cannabinoid-related receptor CB2R and TRPV1 and spinal microglial polarization in rats with primary dysmenorrhea
摘要
Abstract
Objective To observe the effect of electroacupuncture(EA)on the expression of cannabinoid receptor type 2(CB2R),atypical cannabinoid receptor transient receptor potential vanilloid 1(TRPV1),and spinal microglial polarization in rats with primary dysmenorrhea(PDM),so as to explore the underlying mechanism of EA alleviating PDM pain hyperalgesia.Methods Thirty female SD rats were randomly divided into control,model,and EA groups(n=10 rats per group).The PDM model was established by subcutaneous injection of estradiol diphenhydrate combined with intraperitoneal injection of oxytocin.Rats in the EA group were treated with EA(50 Hz)at"Guanyuan"(CV4)and bilateral"Sanyinjiao"(SP6)for 20 min,once a day for 10 consecutive days.The number of writhing,writhing score,and writhing latency were observed.The uterine histopathological changes were observed by H.E.staining.The contents of prostaglandin F2α(PGF2α),prostaglandin E2(PGE2)in serum and interleukin-6(IL-6),interleukin-1β(IL-1β),tumor necrosis factor-α(TNF-α)and interleukin-10(IL-10)in spinal cord were detected by ELISA.The protein expressions of CB2R and TRPV1 in spinal cord and uterus,and inducible nitric oxide synthase(iNOS)and arginase-1(Arg1)in spinal cord were detected by Western blot.Results Compared with the control group,the number and score of writhing,pathological injury score,contents of PGF2α and ratio of PGF2α/PGE2 in serum and IL-1β,TNF-α and IL-6 contents in spinal cord,the protein expressions of TRPV1 in both uterine and spinal cord,and iNOS in spinal cord were significantly increased in the model group(P<0.001),while contents of PGE2 in serum and IL-10 in spinal cord,the expression levels of CB2R in both uterine and spinal cord,and Arg1 in spinal cord were significantly decreased(P<0.001).In comparison with the model group,the number of writhing,pathological injury score,contents of PGF2α and ratio of PGF2α/PGE2 in serum and IL-1β,TNF-α and IL-6 contents in spinal cord,the protein expressions of TRPV1 in both uterine and spinal cord and iNOS in spinal cord were obviously decreased(P<0.001,P<0.05,P<0.01),whereas the writhing latency was considerably prolonged(P<0.05),with elevated contents of PGE2 in serum and IL-10 in spinal cord,and elevated protein expression levels of CB2R in both uterine and spinal cord,and Arg1 in spinal cord(P<0.01,P<0.001)in the EA group.H.E.staining showed degeneration,swelling,and necrosis of endometrial epithelial cells,dilation of glands,stromal hyperemia,and granulocyte infiltration in the model group,which were relatively milder in the EA group.Conclusion EA can alleviate uterine inflammatory response,pathological damage and pain sensitization in PDM rats,which may be related to activating peripheral and central CB2R expression,inhibiting TRPV1 expression,promoting spinal cord microglia polarization from M1 to M2,thus reducing neuroinflammation.关键词
原发性痛经/电针/痛觉敏化/大麻素受体2/瞬时感受器电位香草酸亚型1/小胶质细胞/神经炎性反应Key words
Primary dysmenorrhea/Electroacupuncture/Pain hyperalgesia/Cannabinoid receptor type 2/Transient receptor potential vanilloid 1/Microglia/Neuroinflammation引用本文复制引用
夏飞雁,刘余,钟艳,何溪沁冰,余伊琳,李涵霖,张欣怡..电针对原发性痛经大鼠大麻素相关受体CB2R、TRPV1及脊髓小胶质细胞极化的影响[J].针刺研究,2025,50(11):1248-1256,9.基金项目
国家自然科学基金项目(No.82004490) （No.82004490）
湖南省自然科学基金项目(No.2025JJ50660) （No.2025JJ50660）
湖南省教育厅科学研究重点项目(No.24A0268) （No.24A0268）
湖南省普通高校创新创业教育中心项目(No.30) （No.30）
长沙市自然科学基金项目(No.kq2502163) （No.kq2502163）
湖南中医药大学研究生创新项目(No.2025CX065) （No.2025CX065）
