针刺研究2025，Vol.50Issue(11)：1286-1296,11.DOI:10.13702/j.1000-0607.20240546
基于miR-34a/sirt1/p53信号通路探讨温和灸对血管和肾脏的抗衰作用
Mild moxibustion attenuates vascular and renal aging in senescent rats by regulating the miR-34a/sirt1/p53 signaling pathway
摘要
Abstract
Objective To observe the effect of mild moxibustion on microRNA-34a(miR-34a)/silent information regulator 1(sirt1)/tumor protein p53(p53)signaling pathway in the thoracic aorta and kidney of senescent rats,so as to investigate the mechanism of mild moxibustion in attenuating vascular and renal aging.Methods Fifty 8-month-old male SD rats were given intraperitoneal injection of D-galactose(300 mg·kg-1·d-1)for 4 weeks to establish aging model.Twenty-four successfully modeled rats were randomly divided into aged control,medication and mild moxibustion groups(with 8 rats in each group),with 8 additional 3-month-old male SD rats as the young control group.Rats of the mild moxibustion group received mild moxibustion at"Guanyuan"(CV4)and bilateral"Shenshu"(BL23)once daily for 5 d/week over 8 weeks.Rats of the medication group were intraperitoneally injected with testosterone propionate(7 mg/kg)every 3 d for 8 weeks.The aged and young control groups received equivalent volumes of 0.9%normal saline.Exhaustive swimming time was measured pre-and post-treatment.Histomorphology and collagen content of the thoracic aorta and kidney were assessed using HE and Masson staining.Serum total testosterone(TT)and free testosterone(FT)contents were detected by ELISA.Klotho protein expression in aortic and renal tissue was detected via immunohistochemistry.The relative protein expressions of sirt1,p53,and transforming growth factor-β1(TGF-β1)were detected using Western blot,while real-time PCR was used to detect miR-34a,sirt1,and p53 mRNA expressions.Results Compared with the young control group,the exhaustive swimming time of rats in the aged control group shortened(P<0.01),collagen deposition in aortic and renal tissues increased(P<0.01),serum TT/FT content reduced(P<0.01),the positive expression of Klotho,the protein and mRNA expression levels of sirt1 in aortic and renal tissue decreased(P<0.01),and the expression levels of p53 protein and mRNA,TGF-β1 protein,and miR-34a mRNA increased(P<0.01).Mild moxibustion and medication interventions reversed these trends:both groups showed prolonged swimming time(P<0.01),reduced collagen(P<0.01),elevated serum TT/FT content(P<0.01),and down-regulated p53 protein,miR-34a mRNA in aortic and renal tissue(P<0.01),TGF-β1 protein and p53 mRNA in aortic tissue(P<0.05,P<0.01),and mRNA expression level of sirt1 in renal tissue(P<0.01).Compared with the medication group,the collagen deposition of aortic tissue in the mild moxibustion group decreased(P<0.05),positive expression of Klotho in renal tissue,and protein expression level of sirt1 in aortic tissue increased(P<0.05),the protein expression level of p53 in renal tissue decreased(P<0.05),while the mRNA expression levels of p53 in aortic tissue and miR-34a in renal tissue decreased(P<0.05).HE staining showed thickened thoracic aorta and reduced glomeruli number in the aged control group,which was improved in the medication and mild moxibustion groups,including thinner thoracic aortic intima,more complete glomerular structure,and more regular renal icles and glomerular lumen,and the improvement in the mild moxibustion group was better than that in the medication group.Conclusion Mild moxibustion exerts anti-aging effects on the thoracic aorta and kidney of senescent rats,which may be related with its effect on modulating the miR-34a/sirt1/p53 signaling pathway.关键词
温和灸/衰老/miR-34a/sirt1/p53信号通路Key words
Mild moxibustion/Aging/miR-34a/sirt1/p53 signaling pathway引用本文复制引用
李怡,李学智,何昕璐..基于miR-34a/sirt1/p53信号通路探讨温和灸对血管和肾脏的抗衰作用[J].针刺研究,2025,50(11):1286-1296,11.基金项目
重庆市基础科学与前沿技术专项项目(No.cstc2017jcyjAX0213) （No.cstc2017jcyjAX0213）
