中国水稻科学2025，Vol.39Issue(6)：711-730,20.DOI:10.16819/j.1001-7216.2025.241005
植物引导编辑技术——作物育种的新方向
Plant Prime Editing:A New Direction in Crop Breeding
摘要
Abstract
Plant Prime Editing(PPE)provides a novel approach for precise genome modification in plants,overcoming the limitations of conventional gene editing methods that rely on double-strand breaks and exogenous donor DNA.It enables not only arbitrary base substitutions,but also precise insertions and deletions of small to large DNA fragments,with reduced off-target effects.This review systematically summarizes the latest research advances in PPE technology globally,including its development history and working mechanism,applications in crop breeding and trait improvement,and representative cases.It also discusses the expansion of prime editing into large-scale genome editing and multiplex gene editing.To address the bottlenecks faced by PPE systems in plant genetic transformation,several potential solutions are proposed.Finally,the broad application prospects of PPE technology in plant genetic improvement and its potential integration with artificial intelligence(AI)in future research are outlined.关键词
植物引导编辑技术/CRISPR/Cas/精准编辑/作物育种/植物遗传转化Key words
plant prime editing/CRISPR/Cas/precision gene editing/crop breeding/plant genetic transformation引用本文复制引用
宋安琪,吴松权,马秋月,班宛宁,刘相国,金永梅..植物引导编辑技术——作物育种的新方向[J].中国水稻科学,2025,39(6):711-730,20.基金项目
吉林省农业科技创新工程资助项目(CXGC2024ZD001、CXGC2023RCY050) （CXGC2024ZD001、CXGC2023RCY050）
吉林省自然科学基金资助项目(20210101026JC). （20210101026JC）
