中国水稻科学2025，Vol.39Issue(6)：771-778,8.DOI:10.16819/j.1001-7216.2025.240705
利用CBE系统编辑Wx基因调控稻米直链淀粉含量
Regulation of Amylose Content in Rice by Editing Wx Using CBE Base Editor
摘要
Abstract
[Objective]The Wx gene,a key regulator of amylose synthesis in rice,encodes a granule-bound starch synthase and plays a crucial role in determining the amylose content,thereby directly influencing the quality traits of rice.[Method]We used the CBE base editor to introduce base substitutions within the first exon of the Wx gene in Shendao18 variety,resulting in two distinct mutation types:GCCCCC→GCCCTT and GCCCCC→GCTCTC.Both mutations led to the substitution of proline with leucine at position 94 of the Wx protein,designated as WxP94L-1 and WxP94L-2 respectively.[Result]Field experiments demonstrated that compared to the wild type,both WxP94L-1 and WxP94L-2 plants exhibited no significant differences in plant height,tiller number per plant,panicle length,grain morphology or 1000-grain weight.Rice quality analysis revealed that the amylose content decreased from 14.9%(wild type)to 12.5%(WxP94L-1)and 12.1%(WxP94L-2),while traits such as viscosity,alkali spreading value and protein content showed no significant change compared to the wild type.[Conclusion]Our study successfully generated a novel allele of the Wx gene named WxP94L using CBE base editor which resulted in a slight reduction in amylose content;thus providing a way for enhancing rice quality.关键词
Wx/直链淀粉含量/单碱基编辑/CBEKey words
Wx/amylose content/base editing/CBE引用本文复制引用
陈玉叶,焦晓真,王健,王克剑,陈峰,朱克明,刘朝雷..利用CBE系统编辑Wx基因调控稻米直链淀粉含量[J].中国水稻科学,2025,39(6):771-778,8.基金项目
浙江省自然科学基金资助项目(LQ20C130010) （LQ20C130010）
国家水稻产业技术体系(山东省农业良种工程)资助项目(2024LZGCQY007) （山东省农业良种工程）
山东省科技型中小企业创新能力提升工程资助项目(2024TSGC0841) （2024TSGC0841）
山东省水稻产业技术体系资助项目(SDAIT-17-17). （SDAIT-17-17）
