中国水稻科学2025，Vol.39Issue(6)：779-788,10.DOI:10.16819/j.1001-7216.2025.250208
水稻OsRDR5基因功能及作用机制初步解析
Preliminary Analysis of Function and Mechanism of OsRDR5 Gene in Rice
摘要
Abstract
[Objective]Drought is a major constraint on rice production,and drought avoidance is the most important mechanism for drought resistance of crops.Understanding the mechanism of OsRDR5,a candidate gene of drought tolerance in rice,can provide a theoretical basis for the improvement of drought tolerance in rice.[Method]The nucleotide sequence,protein domain and phylogenetic relationship of RDR5 were analyzed using the NCBI database.The tissue expression pattern was analyzed by qRT-PCR.An OsRDR5 gene knockout mutant was constructed by the CRISPR/Cas9 system.The effect of OsRDR5 on rice deep root ratio was evaluated by the"basket method".[Result]OsRDR5 has a Coa3_cc domain,belonging to the cytochrome c oxidase assembly factor 3 gene family.OsRDR5 is located in the endoplasmic reticulum and is expressed in roots,stems and leaves of rice.Phenotypic identification of deep root ratio showed that the wild type had a deep root ratio of 53.7%,while the mutant lines had a deep root ratio of 63.3%and 64.9%,respectively.The deep root ratio of the mutant was significantly increased.A total of 1434 differentially expressed genes were identified by root transcriptomic analysis.GO enrichment analysis revealed significant enrichment of these genes in pathways related to drought stress,including response to water deprivation,abscisic acid and reactive oxygen species,among which several NAC genes(OsNAC016,OsNAC45,etc.)related to drought stress were up-regulated in the water deprivation pathway.[Conclusion]OsRDR5 knockout affects the deep root ratio of rice and plays an important role in drought avoidance of rice.关键词
水稻/OsRDR5/基因功能/避旱性Key words
rice/OsRDR5/gene function/drought avoidance引用本文复制引用
侯桂花,周立国,雷建国,陈虹,聂元元..水稻OsRDR5基因功能及作用机制初步解析[J].中国水稻科学,2025,39(6):779-788,10.基金项目
江西省农业科学院基础研究与人才培养项目(3031479) （3031479）
江西省财政厅单产提升项目(3032467) （3032467）
赣鄱俊才支持计划资助项目(20243BCE51058) （20243BCE51058）
水稻种质创新与育种江西省重点实验室项目 （）
农业农村部粮食作物基因资源评价利用重点实验室开放课题. （）
