中国中药杂志2025，Vol.50Issue(20)：5684-5696,13.DOI:10.19540/j.cnki.cjcmm.20250710.202
基于UHPLC指纹图谱和网络药理学的云南独蒜兰"异病同治"肝脏炎症、阿尔茨海默病的质量标志物研究
Study on quality markers of Pleione yunnanensis for"same treatment for different diseases"in liver inflammation and Alzheimer's disease based on UHPLC fingerprint and network pharmacology
摘要
Abstract
This study aims to screen quality markers(Q-markers)of Pleione yunnanensis for the treatment of liver inflammation and Alzheimer's disease(AD)based on the concept of"same treatment for different diseases"using ultra-high-performance liquid chromatography(UHPLC)fingerprinting combined with network pharmacology and molecular docking,and to perform quantitative analysis of the identified Q-markers.The UHPLC fingerprints of 15 batches of P.yunnanensis were established and subjected to similarity evaluation,cluster analysis(CA),principal component analysis(PCA),and orthogonal partial least squares discriminant analysis(OPLS-DA)to identify characteristic components responsible for quality variations.Network pharmacology and molecular docking were employed to explore the potential active components,related targets,and signaling pathways underlying the"same treatment for different diseases"mechanism for liver inflammation and AD.Based on the criteria of effectiveness,specificity,and measurability,Q-markers of P.yunnanensis were identified and quantified.Ten common peaks were identified in the UHPLC fingerprints of 15 batches,with eight components identified.Similarity scores ranged from 0.774 to 0.987.Chemical pattern recognition analysis identified that shancigusin H,militarine,and gymnoside V were the major characteristic components contributing to quality differences among batches.Results of network pharmacology and molecular docking revealed that dactylorhin A and militarine were the key active components exerting anti-inflammatory and neuroprotective effects.These components acted on 13 core targets,including SRC,CASP3,and CTNNB1,and regulated signaling pathways such as the PI3K-Akt signaling pathway and arachidonic acid metabolism.Based on the effectiveness,specificity,and measurability of Q-marker,dactylorhin A and militarine were selected as the Q-markers of P.yunnanensis.Quantitative analysis demonstrated that the content of dactylorhin A ranged from 0.527％to 2.21％,and militarine ranged from 0.731％to 2.58％across the 15 batches.The UHPLC fingerprint method and Q-marker-based quantification approach are robust and reliable,providing experimental evidence for quality control and standardization of P.yunnanensis.关键词
云南独蒜兰/指纹图谱/网络药理学/分子对接/阿尔茨海默病/肝脏炎症/含量测定Key words
Pleione yunnanensis/fingerprint/network pharmacology/molecular docking/Alzheimer's disease/liver inflammation/content determination引用本文复制引用
张元现,黄勇,李月婷,王静,陈轻轻,张金玲,王倩,龙亚丽,周思佳,巩仔鹏,郑林..基于UHPLC指纹图谱和网络药理学的云南独蒜兰"异病同治"肝脏炎症、阿尔茨海默病的质量标志物研究[J].中国中药杂志,2025,50(20):5684-5696,13.基金项目
国家自然科学基金项目(82360765) （82360765）
贵州省科技计划项目(黔科合平台人才-CXTD[2023]019) （黔科合平台人才-CXTD[2023]019）
贵州省高层次创新型人才项目(黔科合平台人才-GCC[2023]036,黔科合平台人才-GCC[2022]031-1) （黔科合平台人才-GCC[2023]036,黔科合平台人才-GCC[2022]031-1）
贵州医科大学培育项目(20NSP072) （20NSP072）
