中国中药杂志2025，Vol.50Issue(20)：5706-5716,11.DOI:10.19540/j.cnki.cjcmm.20250604.101
基于LC-MS结合多元统计分析的栽培地黄与野生地黄中代谢产物特征分析
Metabolite characteristics analysis of cultivated and wild Rehmannia glutinosa based on LC-MS combined with multivariate statistical analysis
摘要
Abstract
To analyze the difference of overall metabolites in cultivated and wild Rehmannia glutinosa,this study used cultivated and wild R.glutinosa from different locations as research objects.Their metabolites were detected using liquid chromatography-mass spectrometry(LC-MS),and multivariate statistical analysis was adopted to analyze metabolites.Differential metabolites were identified,and differential metabolic pathways were determined based on the Kyoto Encyclopedia of Genes and Genomes(KEGG)database.The results showcased that 1 802 metabolites were detected,and the metabolites of cultivated and wild R.glutinosa were similar,including 13 kinds of compounds such as terpenoids,phenolic acids,lipids,and alkaloids.Overall,the relative content of metabolites of wild R.glutinosa was higher than that of cultivated R.glutinosa.There were 665 differential metabolites between cultivated and wild R.glutinosa.A total of 157 differential metabolites were up-regulated,and 508 differential metabolites were down-regulated.According to the KEGG database,93 metabolites with significant differences were annotated,with phenolic acids representing the highest proportion.There were a total of 55 metabolic pathways corresponding to differential metabolites,which were divided into three categories:metabolism,genetic information processing,and environmental information processing.Significantly different pathways included flavonoid biosynthesis(ko00941),phenylpropane biosynthesis(ko00940),and pyrimidine metabolism(ko00240),all of which were associated with plant metabolism.The study found that the metabolite species of wild and cultivated R.glutinosa were consistent,whereas the overall metabolite content of wild R.glutinosa was higher than that of cultivated R.glutinosa,and the difference in their pharmacological effects remained to be further investigated.关键词
野生地黄/栽培地黄/不同产地/代谢物/代谢通路Key words
wild Rehmannia glutinosa/cultivated Rehmannia glutinosa/different locations/metabolites/metabolic pathway引用本文复制引用
陈亚曼,王丰青,谢彩侠,刘蕊,王庆婷,张娟,苏成福,龚海燕,刘庆普,雷敬卫,杨春静..基于LC-MS结合多元统计分析的栽培地黄与野生地黄中代谢产物特征分析[J].中国中药杂志,2025,50(20):5706-5716,11.基金项目
国家自然科学基金项目(82073952,82373985) （82073952,82373985）
