植物营养与肥料学报2025，Vol.31Issue(10)：1970-1982,13.DOI:10.11674/zwyf.2024636
腐植酸尿素高效促进秸秆分解的微生物群落响应机制
Response mechanism of microbial community that efficiently promotes straw decomposition through humic acid urea application
摘要
Abstract
[Objectives]This study explored the effect of humic acid urea(HAU)application on the decomposition process of maize straw and identified the key differential microbial groups involved in the decomposition of straw,aiming to provide nutrient management method for the efficient utilization of straw resources in North China Plain.[Methods]The experiment was carried out in Modern Agricultural Research and Development Base of Henan Province,where a long-term experiment of new fertilizers was conducted since 2016.In October 2022 after winter wheat sowing,maize straw bags were buried into the urea and humic acid urea treatment plots,respectively,and straw bags were collected at different burying days during the wheat season(200 days in total)to determine the residual straw weight,the organic carbon and nitrogen content,enzyme activity,bacterial and fungal diversity.[Results]Compared with the urea treatment,HAU accelerated the decomposition process of maize straw,increased the decomposition rates,nitrogen and carbon release rate of straw by 6.98%,32.29%,and 6.56%,respectively.It also enhanced the release rate of cellulose,hemicellulose and lignin by 12.27%,9.35%and 14.27%,respectively.The activities of β-glucosidase,cellobiose hydrolase,leucine aminopeptidase and laccase were also improved.The dominant bacteria involved in straw decomposition were Proteobacteria,Bacteroidota,Actinobacteriota and Firmicutes.And the dominant fungi were Ascomycota,Mucoromycota and Basidiomycota.HAU application enriched bacterial groups included Agromyces,Microterricola,Paenarthrobacter,Pseudomonas,Chryseobacterium,Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium,and Mesorhizobium,etc.And the enriched fungal groups included Actinomucor,Hannaella,Trichocladium,Aspergillus,etc.Straw N content,lignin/N ratio and β-glucosidase activity were the most important factors affecting the community structure of straw bacteria and fungi.[Conclusions]The long-term application of humic acid urea proves to be more advantageous than the use of common urea in fostering more diverse bacteria and fungi species associated with straw decomposition across various stages.This includes the proliferation of distinct nitrogen-fixing bacteria such as Paenarthrobacter,Allorhizobium-Neorhizobium-Pararhizobium-Rhizobium,and so on,as well as enhancing the activities of enzymes responsible for carbon and nitrogen transformation.Consequently,this accelerates the decomposition process of maize straw.Therefore,the application of humic acid urea emerges as an effective strategy for achieving efficient utilization of straw nutrient resources in the North China Plain.关键词
腐植酸尿素/秸秆分解率/秸秆碳氮转化酶/秸秆分解微生物/差异微生物Key words
humic acid urea/straw decomposition rate/straw C/N-transformation enzymes/microbes involved in straw decomposition/differential taxa引用本文复制引用
郭腾飞,岳克,宋晓,丁世杰,罗鹏,徐新朋,黄绍敏,张倩,岳艳军..腐植酸尿素高效促进秸秆分解的微生物群落响应机制[J].植物营养与肥料学报,2025,31(10):1970-1982,13.基金项目
河南省农业科学院优秀青年科技基金项目(2024YQ04) （2024YQ04）
国家自然科学基金项目(32202603) （32202603）
农田智慧施肥项目(20221805). （20221805）
