中药材2025，Vol.48Issue(2)：315-319,5.DOI:10.13863/j.issn1001-4454.2025.02.009
药用蜘蛛棒毛络新妇及其混淆品的分子鉴定
Molecular Identification of the Medicinal Spider Trichonephila clavata and Its Adulterants
摘要
Abstract
Objective:To establish a molecular identification method that can accurately identify Trichonephila clavata and its con-fused products to solve the confusion of medicinal materials and the hidden danger of drug use caused by the diversity of spider species.Methods:DNA was extracted from 70 samples,COⅠ sequence characteristics were analyzed,intraspecious and interspecific genetic dis-tances were calculated,and ML phylogenetic trees were constructed to evaluate the validity of COⅠ sequence as an identification meth-od of Trichonephila clavata and its confounding products.R language mapping software was used to convert molecular barcodes into QR codes,establish a dedicated DNA barcode library,and verify 8 commercially available spiders.Results:The length of COⅠ sequence of Trichonephila clavata was 603 bp,the content of GC was 31.8%to 32.5%,there were 16 base variation sites,and there were 14 haplo-types(Hd=0.8236),indicating that the sample population had rich diversity.The average interspecific genetic distance(0.1685)was significantly greater than the average intrasecific genetic distance(0.0034),which was consistent with the DNA Barcoding Gap test re-sults,indicating that there was a certain DNA barcode gap between COⅠ sequence and other species.In the constructed phylogenetic tree,different species were grouped into single branches.Among the 8 commercial samples verified by actual measurement,6 samples were identified as Trichonephila clavata,and 2 counterfeits were also accurately identified,with an accuracy of 100%.Conclusion:The molecular identification method of COⅠ sequence established in this study is highly specific,and can effectively solve the species iden-tification problem of the complete herbal medicine and its powder of Trichonephila clavata.关键词
棒毛络新妇/花蜘蛛/真伪鉴别/细胞色素C氧化酶Ⅰ/DNA条形码Key words
Trichonephila clavata L.Koch/Trichonephila clavata L.Koch with red,green,yellow or others stripes/Authenticity identification/Cytochrome C oxidase Ⅰ/DNA barcode分类
中医学引用本文复制引用
蔡文正,杨梦慧,张蒙蒙,张梦颖,张成桂,徐士奎,杨自忠..药用蜘蛛棒毛络新妇及其混淆品的分子鉴定[J].中药材,2025,48(2):315-319,5.基金项目
国家自然科学基金项目(32160113) （32160113）
云南省科技厅基础研究专项-重点项目(202301AS070083) （202301AS070083）
云南省重大科技专项计划项目(202002AA100007) （202002AA100007）
云南省"兴滇英才支持计划"团队项目(202305AS350001) （202305AS350001）
