中药材2025，Vol.48Issue(2)：462-467,6.DOI:10.13863/j.issn1001-4454.2025.02.031
绿原酸调控AMPK/SIRT1通路对ox-LDL诱导的泡沫细胞自噬及炎症反应的影响
Effects of Chlorogenic Acid on Autophagy and Inflammatory Response of ox-LDL-Induced Foam Cells by Regulating AMPK/SIRT1 Pathway
摘要
Abstract
Objective:To investigate the regulatory effects of chlorogenic acid on autophagy and inflammation in ox-LDL-induced foam cells through AMPK/SIRT1 signaling pathway.Methods:RAW264.7 macrophages were divided into blank control group,model group,chlorogenic acid(low,medium and high concentration)groups,chlorogenic acid high concentration with Compound C group.The degree of foam cells was observed by oil red O staining.The effect of chlorogenic acid on the proliferation of bubble cells was observed by MTT method.The autophagosomes were observed by transmission electron microscopy.The contents of TNF-α,IL-6 and IL-10 were detected by ELISA.The mRNA expressions of AMPK,SIRT1,Beclin1 and P62 were detected by fluorescence quantitative PCR.The protein expressions of SIRT1,ATG16L1 and P62 were detected by immunofluorescence labeling.Results:Oil red O staining showed that foam cells were successfully prepared after ox-LDL treatment.Compared with the model group,the number of autophagosomes in-creased,the secretion of TNF-α and IL-6 decreased,the secretion of IL-10 increased,the mRNA expressions of AMPK,SIRT1,Beclin1 and the protein expressions of SIRT1,ATG16L1 increased,and the mRNA and protein expressions of P62 decreased in each chlorogenic acid concentration group.Compound C could reduce the effect of chlorogenic acid.Conclusion:Chlorogenic acid can induce autophagy of bubble cells by regulating AMPK/SIRT1 pathway,down-regulate the overexpression of TNF-α and IL-6,and up-regulate the expression of IL-10,which may be one of the anti-AS mechanisms of chlorogenic acid.关键词
绿原酸/泡沫细胞/自噬/动脉粥样硬化/炎症反应Key words
Chlorogenic acid/Foam cells/Autophagy/Atherosclerosis/Inflammatory response分类
医药卫生引用本文复制引用
于红红,姚佳倩,杨韵琪,罗培,俞琦,马玉玲,田维毅..绿原酸调控AMPK/SIRT1通路对ox-LDL诱导的泡沫细胞自噬及炎症反应的影响[J].中药材,2025,48(2):462-467,6.基金项目
国家自然科学基金资助项目(81860779) （81860779）
贵州省科技厅项目(黔科合基础ZK[2021]549) （黔科合基础ZK[2021]549）
贵州省卫生健康委科技项目([2021]108) （[2021]108）
贵州中医药大学研究生教育创新计划项目(YCXKYB2023012) （YCXKYB2023012）
