南方医科大学学报2025，Vol.45Issue(11)：2330-2339,10.DOI:10.12122/j.issn.1673-4254.2025.11.05
豨莶丸治疗类风湿关节炎的分子机制:基于蛋白质组学
Molecular mechanism of Xixian Pills for improving rheumatoid arthritis in rats:a proteomic analysis
摘要
Abstract
Objective To analyze the molecular mechanism of Xixian Pills for treatment of rheumatoid arthritis(RA).Methods Forty-eight rats were randomized into 6 groups(n=8),including a normal control group,a collagen-induced arthritis(CIA)model group,3 Xixian Pills treatment(200,400 and 800 mg/kg)groups,and a Tripterygium glycosides tablet(TGT)treatment group.In the latter 4 groups,the rats were treated with daily gavage of Xixian Pills or TGT 2 weeks after CIA modeling for 3 consecutive weeks.The differentially expressed proteins in high-dose Xixian Pills group and the model group compared with the normal control group were screened based on the tandem mass spectrometry tag(TMT)technology,and the core targets and signaling pathways were analyzed.The immune cell infiltration and gene expression data were analyzed using ggplot2 and tidyverse packages,and the correlation coefficients between the core targets and the immune cells were calculated.Results The CIA rats showed significantly increased serum levels of TNF-α and IL-6 and lowered serum IL-10 level.Treatments with high-and medium-dose Xixian Pills and TGT all significantly reduced serum TNF-α and IL-6 and increased IL-10 levels in CIA rats.Proteomic analysis identified 160 differential proteins between the model group and high-dose Xixian Pills group,and the core targets included CCL5,STAT1,GZMB and IL7R.The areas under the ROC curve of CCL5 and STAT1 were both greater than 0.9.Immunohistochemical and immunofluorescence staining revealed increased levels of CCL5 and STAT1 in the ankle joints of CIA rats,which were significantly decreased after treatment with Xixian Pills.Conclusion Treatment with Xixian Pills offers protection of the joints in CIA rats possibly by inhibiting joint inflammation via regulating protein expressions of CCL5 and STAT1.关键词
豨莶丸/类风湿关节炎/蛋白质组学/免疫细胞浸润Key words
Xixian Pills/rheumatoid arthritis/proteomics/immune cell infiltration引用本文复制引用
李亚辉,杨欣,姚血明,黄聪..豨莶丸治疗类风湿关节炎的分子机制:基于蛋白质组学[J].南方医科大学学报,2025,45(11):2330-2339,10.基金项目
贵州省科技厅贵州省基础研究计划项目(黔科合基础-ZK[2024]一般358) （黔科合基础-ZK[2024]一般358）
贵州中医药大学国家与省级科技创新人才团队培育项目(贵中医TD合字[2022]004号) （贵中医TD合字[2022]004号）
