南方医科大学学报2025，Vol.45Issue(11)：2375-2384,10.DOI:10.12122/j.issn.1673-4254.2025.11.10
电针通过Bcl-2/Bax/caspase-3信号通路修复海马线粒体损伤改善创伤后应激障碍大鼠的焦虑症状
Electroacupuncture improves post-traumatic stress disorder in rats by alleviating hippocampal mitochondrial injury via regulating Bcl-2/Bax/caspase-3 signaling
摘要
Abstract
Objective To investigate the mechanism underlying the therapeutic effect of electroacupuncture(EA)on post-traumatic stress disorder(PTSD)in rats.Methods Forty male SD rats were randomized equally into blank control group,PTSD model group,sham-acupuncture group,paroxetine group,and EA group.In the latter 3 groups,the rat models of PTSD,induced by continuous single-prolonged stress and plantar electrical stimulation,were treated with EA at GV20,GV24,BL18 and BL23 acupoints for 15 min(5 times a week for 3 weeks),sham-acupuncture without electrical stimulation,or gavage with paroxetine suspension on the same schedule.Behavioral changes of the rats were evaluated using open field test(OFT)and elevated plus maze(EPM)test.Hippocampal pathologies and neuronal changes were examined with HE and Nissl staining,and mitochondrial ultrastructure was examined using electron microscopy.The mRNA and protein expression levels of Bcl-2,Bax,and caspase-3 were detected by RT-qPCR and immunofluorescence staining.Results The rat models of PTSD showed significantly reduced total distance traveled in OFT and distance and time spent in the open arms of the EPM,with decreased hippocampal neurons,obvious neuronal and mitochondrial pathologies,decreased hippocampal expression of Bcl-2,and increased Bax and caspase-3 expressions.Treatments with paroxetine and EA both significantly improved behavioral changes of the rat models,increased the number of Nissl-stained neurons,obviously alleviated pathologies in the hippocampal neurons and mitochondrial ultrastructure,increased hippocampal Bcl-2 expression,and lowered caspase-3 expressions.Paroxetine showed significantly better effect than EA for improving performance of the rats in EPM test,whereas sham-acupuncture did not produce any significant improvement.Conclusion EA alleviates PTSD in rats possibly by upregulating Bcl-2 and downregulating Bax and caspase-3,thereby ameliorating hippocampal mitochondrial damage.关键词
创伤后应激障碍/电针/线粒体/神经细胞/caspase-3/焦虑障碍Key words
post-traumatic stress disorder/electroacupuncture/mitochondria/neurons/caspase-3/anxiety disorder引用本文复制引用
马丹丹,程洁,张虹,刘广,宋凯..电针通过Bcl-2/Bax/caspase-3信号通路修复海马线粒体损伤改善创伤后应激障碍大鼠的焦虑症状[J].南方医科大学学报,2025,45(11):2375-2384,10.基金项目
国家中医药管理局专项经费(500006007):全国名老中医药专家张虹传承工作室项目 （500006007）
四川省自然科学基金(2023NSFSC1821) （2023NSFSC1821）
